Immunoprecipitation

This immunoprecipitation protocol details individual steps for the enrichment and purification process of specific proteins from a complex cell lysate using an antibody bound to a solid matrix. Purified antigen(s) can be eluted by various methods, and the resultant protein target can be analyzed and...

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Bibliographic Details
Published inCold Spring Harbor protocols Vol. 2017; no. 12; p. pdb.prot098640
Main Authors DeCaprio, James, Kohl, Thomas O
Format Journal Article
LanguageEnglish
Published United States 01.12.2017
Subjects
Antigens - isolation & purification
Immunoprecipitation - methods
Proteins - isolation & purification
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Summary:This immunoprecipitation protocol details individual steps for the enrichment and purification process of specific proteins from a complex cell lysate using an antibody bound to a solid matrix. Purified antigen(s) can be eluted by various methods, and the resultant protein target can be analyzed and/or identified by numerous assays, including the enzyme-linked immunosorbent assay (ELISA), western blotting, or mass spectrometry.
ISSN:1559-6095
DOI:10.1101/pdb.prot098640