ASSOCIATION OF GENETIC POLYMORPHISM AND EXPRESSION OF UMOD GENE AND CHRONIC KIDNEY DISEASE

• Published in: Wiadomości lekarskie (1960) Vol. 74; no. 9 cz 2; p. 2297
• Main Authors: Hasan, Farah Thamer, Mohey, Mahmood Ahmed
• Format: Journal Article
• Language: English
• Published: Poland 2021
• Subjects: Adult; Aged; Aged, 80 and over; Female; Genotype; Humans; Male; Middle Aged; Polymorphism, Single Nucleotide; Renal Insufficiency, Chronic - genetics; Uromodulin - genetics; Uromodulin protein; Chronic Kidney disease; genetic polymorphism
• DOI: 10.36740/WLek202109209
• ISSN: 0043-5147

The aim: This study is designed to investigate the possible association of genetic polymorphism expression of UMOD gene and chronic kidney disease in population. Materials and methods: In the current study, the single-nucleotide polymorphisms (SNPs) were genotyped in the promoter region of the UMOD gene in CKD patients to assess its association with the kidney outcome of CKD. So 50 patients' blood samples suffered from CKD were collected. Among these patients, 21 were men and 29 women, aged 35 - 85 years old. Another group included 50 healthy subjects. DNA was extracted from all blood samples with EDTA using Quick DNA miniprep Kit ZYMO, (Cat№ D3025) according to manufacturer's instructions. Genotyping of 1 common polymorphisms (rs4293393) of the UMOD gene was done and the RNA was extracted and converted to cDNA and a set of primers was used to amplify specific region within the UMOD gene; another set was used to amplify the GAPDH gene to use it in calculation as a reference gene. Results and conclusions: After statistical analysis, the results showed that there could be association between having CC mutant polymorphism in UMOD gene and having CKD.