Design of Experiment (DoE) for Optimization of HPLC Conditions for the Simultaneous Fractionation of Seven α-Amylase/Trypsin Inhibitors from Wheat (Triticum aestivum L.)

• Published in: Processes Vol. 10; no. 2; p. 259
• Main Authors: Sagu, Sorel Tchewonpi, Huschek, Gerd, Braga, Tess Waldbach, Rackiewicz, Michal, Homann, Thomas, Rawel, Harshadrai M.
• Format: Journal Article
• Language: English
• Published: Basel MDPI AG 01.02.2022
• Subjects: Amylases; Anion exchanging; Chemicals; Chloroform; Chromatography; Design of experiments; Design optimization; Efficiency; Fractionation; High performance liquid chromatography; Ion exchange; Proteins; Proteomics; Response surface methodology; Trypsin; Trypsin inhibitors; Wheat; α-Amylase; Germany

Wheat alpha-amylase/trypsin inhibitors remain a subject of interest considering the latest findings showing their implication in wheat-related non-celiac sensitivity (NCWS). Understanding their functions in such a disorder is still unclear and for further study, the need for pure ATI molecules is on...