ENZYME CONDUCTOMETRIC BIOSENSOR FOR FRUCTOSE DETERMINATION

The conductometric biosensor for fructose determination based on fructose dehydrogenase and potassium ferricyanide mediator as electron acceptor has been developed. The enzyme was immobilized on a surface of the conductometric transducer together with bovine serum albumin using crosslinking with glu...

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Published inBiotechnologia acta Vol. 6; no. 3; pp. 46 - 52
Main Authors Dudchenko, O Y, Pyeshkova, V N, Soldatkin, O O, Soldatkin, O P, Dzyadevych, S V
Format Journal Article
LanguageEnglish
Published Kyiv Palladin Institute of Biochemistry of National Academy of Sciences of Ukraine 01.01.2013
National Academy of Sciences of Ukraine, Palladin Institute of Biochemistry
Subjects
conductometric biosensor
fructose
fructose dehydrogenase
potassium ferricyanide
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Abstract The conductometric biosensor for fructose determination based on fructose dehydrogenase and potassium ferricyanide mediator as electron acceptor has been developed. The enzyme was immobilized on a surface of the conductometric transducer together with bovine serum albumin using crosslinking with glutaraldehyde. Working conditions of the discribed fructose biosensor were optimized. The results concerning influence of the buffer solution concentration and potassium ferricyanide concentration on the biosensor performance are given. The fructose biosensor is characterized by high signal reproducibility and selectivity to fructose. The developed conductometric biosensor can be successfully used for fructose monitoring in the procedures of food and clinical diagnostic.
AbstractList The conductometric biosensor for fructose determination based on fructose dehydrogenase and potassium ferricyanide mediator as electron acceptor has been developed. The enzyme was immobilized on a surface of the conductometric transducer together with bovine serum albumin using crosslinking with glutaraldehyde. Working conditions of the discribed fructose biosensor were optimized. The results concerning influence of the buffer solution concentration and potassium ferricyanide concentration on the biosensor performance are given. The fructose biosensor is characterized by high signal reproducibility and selectivity to fructose. The developed conductometric biosensor can be successfully used for fructose monitoring in the procedures of food and clinical diagnostic.
Author Soldatkin, O P
Pyeshkova, V N
Soldatkin, O O
Dzyadevych, S V
Dudchenko, O Y
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SubjectTerms conductometric biosensor
fructose
fructose dehydrogenase
potassium ferricyanide
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