Process optimization for large-scale production of TGF- ? -PE40 in recombinant Escherichia coli : effect of medium composition and induction timing on protein expression

• Published in: Journal of industrial microbiology & biotechnology Vol. 18; no. 4; pp. 260 - 266
• Main Authors: Lee, C, Sun, W-J, Burgess, B W, Junker, B H, Reddy, J, Buckland, B C, Greasham, R L
• Format: Journal Article
• Language: English
• Published: Fairfax Oxford University Press 01.04.1997
• Subjects: Bacteria; E coli; Enzymes; Escherichia coli; Fermentation; Glycerol; Proteins; Yeasts

The effects of medium composition and induction timing on expression of a chimeric fusion protein TGF-α -PE40 (TP-40) in Escherichia coli strain RR1 were examined using a complex medium at several fermentor scales. Two distinctive phases in E. coli catabolism were identified during fermentation base...