Immunochemical determination of cellular prion proteinin plasma from healthy subjects and patients withsporadic CJD or other neurologic diseases

• Published in: Transfusion (Philadelphia, Pa.) Vol. 41; no. 4; pp. 441 - 448
• Main Authors: Völkel, Dirk, Zimmermann, Klaus, Zerr, Inga, Bodemer, Monica, Lindner, Thomas, Turecek, Peter L., Poser, Sigrid, Schwarz, Hans P.
• Format: Journal Article
• Language: English
• Published: Oxford, UK Blackwell Science Inc 01.04.2001
• Subjects: BSE = bovine spongiform encephalopathy; NSE = neuron-specific enolase; PBST = PBS plus Tween 20; PPP = platelet-poor plasma; PrP = prion protein; PrPres = PrP resistant to protease K; PrPsen = PrP sensitive to protease K; sCJD = sporadic CJD; TBST = Tris-buffered saline with Tween 20; TSE = transmissible spongiform encephalopathy

BACKGROUND: Creutzfeldt‐Jakob disease is thought to be caused by conversion of cellular prion protein (PrP) from its soluble form (PrPsen) to a pathologic form (PrPres). The occurrence of a new variant of CJD has increased the demand for a rapid assay capable of detecting a theoretical risk of trans...