Expression of glycogenes in differentiating human NT2N neurons. Downregulation of fucosyltransferase 9 leads to decreased Lewisx levels and impaired neurite outgrowth

• Published in: Biochimica et biophysica acta. General subjects Vol. 1820; no. 12; pp. 2007 - 2019
• Main Authors: Gouveia, Ricardo, Schaffer, Lana, Papp, Suzanne, Grammel, Nicolas, Kandzia, Sebastian, Head, Steven R., Kleene, Ralf, Schachner, Melitta, Conradt, Harald S., Costa, Júlia
• Format: Journal Article
• Language: English
• Published: Elsevier B.V 01.12.2012
• Subjects: carbohydrates; cell adhesion; cells; Central nervous system; epitopes; fluorescence microscopy; Fucosyltransferase 9; genes; glycoproteins; glycosylation; high performance liquid chromatography; HPLC; humans; immunofluorescence; Lewisx; messenger RNA; microarray technology; microscopy; Neurite outgrowth; Neuron glycosylation; Neuronal differentiation; neurons; peptides; precipitin tests; proteins; regulations; RNA interference; PBS; TBS; HRP; Lewisx; PNGase F; HPAEC-PAD; EDTA; EGTA; BSA; MALDI-TOF MS; SDS-PAGE; HNK-1; Gal; Fuc; HexNAc; Neuronal differentiation; dHex; Fucosyltransferase 9; GlcNAc; Hex; Man; Neuron glycosylation; Lex; Neurite outgrowth

Several glycan structures are functionally relevant in biological events associated with differentiation and regeneration which occur in the central nervous system. Here we have analysed the glycogene expression and glycosylation patterns during human NT2N neuron differentiation. We have further stu...