A Plasmodium chabaudi chabaudi high molecular mass glycoprotein translocated to the host cell membrane by a non-classical secretory pathway

• Published in: European journal of cell biology Vol. 78; no. 3; pp. 186 - 193
• Main Authors: Cruz Moura, Ivan, Pudles, Julio
• Format: Journal Article
• Language: English
• Published: Germany Elsevier GmbH 01.03.1999
• Subjects: Animals; Biological Transport; Brefeldin A - pharmacology; Carbonates; Cell Membrane - metabolism; Erythrocytes - metabolism; Erythrocytes - parasitology; Female; glycoproteins; Glycoproteins - metabolism; Glycoproteins - secretion; Glycosylation; malaria; Mice; Mice, Inbred BALB C; Molecular Weight; Peptides - metabolism; Plasmodium chabaudi - metabolism; Plasmodium chabaudi chabaudi; Protein Synthesis Inhibitors - pharmacology; protein transport; Protozoan Proteins - metabolism; Protozoan Proteins - secretion; Temperature; protein transport; PBS; IRBCs; IFA; PVM; IRBCM; glycoproteins; i.p; malaria; PPM; Plasmodium chabaudi chabaudi; ER; FITC; BFA

We have purified and characterized a novel high molecular mass glycoprotein of P. chabaudi chabaudi (Pc550gp) that is transported to the erythrocyte membrane during the intraerythrocytic cycle. Immuno fluorescence assays with polyclonal monospecific antibodies against Pc550gp show that the protein t...