Three-Dimensional Co-culture of Human Hematopoietic Stem/Progenitor Cells and Mesenchymal Stem/Stromal Cells in a Biomimetic Hematopoietic Niche Microenvironment

• Published in: Methods in molecular biology (Clifton, N.J.) Vol. 2002; p. 101
• Main Authors: Costa, Marta H G, Monteiro, Tiago S, Cardoso, Susana, Cabral, Joaquim M S, Ferreira, Frederico Castelo, da Silva, Cláudia L
• Format: Journal Article
• Language: English
• Published: United States 2019
• Subjects: Biomimetics; Cell Culture Techniques - methods; Cell Proliferation; Cells, Cultured; Coculture Techniques - methods; Fetal Blood - cytology; Hematopoietic Stem Cells - cytology; Humans; Mesenchymal Stem Cells - cytology; Microwells; Hematopoietic stem/progenitor cells (HSPC); Mesenchymal stem/stromal cells (MSC); Co-culture; Hematopoietic niche; Spheroids

The development of cellular therapies to treat hematological malignancies has motivated researchers to investigate ex vivo culture systems capable of expanding the number of hematopoietic stem/progenitor cells (HSPC) before transplantation. The strategies exploited to achieve relevant cell numbers h...