Structure elucidation by one- and two-dimensional 360- and 500-MHz 1H NMR of the oligosaccharide units of two glycoproteins isolated from alveoli of patients with alveolar proteinosis

The structure of the oligosaccharide units of the glycoproteins of M r 36,000 and 62,000 isolated from alveoli of patients with alveolar proteinosis have been determined by one- and two-dimensional 1H NMR spectroscopy at 500 and 360 MHz. Bi-, tri-, and tetraantennary glycans of N-acetyllactosaminic...

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Published inArchives of biochemistry and biophysics Vol. 231; no. 1; pp. 72 - 85
Main Authors Bhattacharyya, Sambhu N., Lynn, William S., Dabrowski, Janusz, Trauner, Karin, Hull, William E.
Format Journal Article
LanguageEnglish
Published United States Elsevier Inc 15.05.1984
Subjects
Chemical Phenomena
Chemistry
Glycoproteins - isolation & purification
Humans
Magnetic Resonance Spectroscopy
Oligosaccharides - isolation & purification
Protons
Pulmonary Alveolar Proteinosis - metabolism
Pulmonary Alveoli - analysis
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Summary:The structure of the oligosaccharide units of the glycoproteins of M r 36,000 and 62,000 isolated from alveoli of patients with alveolar proteinosis have been determined by one- and two-dimensional 1H NMR spectroscopy at 500 and 360 MHz. Bi-, tri-, and tetraantennary glycans of N-acetyllactosaminic type have been found in high percentage. They are 1 → 6 monofucosylated and fully sialylated, the ratio NeuAc(2 → 3) NeuAc × (2 → 6) increasing with increasing degree of branching.
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ISSN:0003-9861
1096-0384
DOI:10.1016/0003-9861(84)90364-3