Fast image-free autofocus method for passive FSPI microscopy

Autofocus is crucial for capturing sharp images with imaging devices for information acquisition. Traditional autofocus strategies based on post-processing become less efficient for passive FSPI microscopy of yet low temporal resolution. In this Letter, a fast and image-free autofocus system is prop...

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Bibliographic Details
Published inOptics letters Vol. 49; no. 11; p. 3110
Main Authors Ni, Mingyang, Cai, Yu, Xue, Yihao, Deng, Huaxia, Gong, Xinglong
Format Journal Article
LanguageEnglish
Published United States 01.06.2024
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Summary:Autofocus is crucial for capturing sharp images with imaging devices for information acquisition. Traditional autofocus strategies based on post-processing become less efficient for passive FSPI microscopy of yet low temporal resolution. In this Letter, a fast and image-free autofocus system is proposed for passive FSPI microscopy. Based on the complementary design of an optical path, the system can measure the focus degree at 5000 fps while maintaining a high light efficiency for imaging. The proposed system can be easily combined with existing trinocular microscopes, which provides a welcomed boost to the practicability of passive FSPI microscopy.
ISSN:1539-4794
DOI:10.1364/OL.516755