Potentiometric biosensor for acrylamide determination in wastewater using wild type amidase from Pseudomonas aeruginosa

• Published in: Transactions on ecology and the environment Vol. 109; pp. 789 - 798
• Main Authors: SILVA, N. A. F, GIL, D, KARMALI, A, MATOS, M
• Format: Conference Proceeding Journal Article
• Language: English
• Published: Southampton WIT 2008; W I T Press
• Subjects: Acrylamide; Acrylic acid; Amidase; Ammonium; Applied sciences; Biological and medical sciences; Biosensors; Biotechnology; Cellulose esters; Cellulose nitrate; Environmental effects; Exact sciences and technology; Fundamental and applied biological sciences. Psychology; Glutaraldehyde; Hazardous areas; Membranes; Methods. Procedures. Technologies; Pollution; Polyethersulfones; Pseudomonas aeruginosa; Reagents; Response time; Substrates; Various methods and equipments; Wastewater; Wastewaters; Water treatment and pollution; Pseudomonadales; Sensitivity analysis; Enzyme; Amidase; Amides; Ion selective electrode; Concentration measurement; Acrylamide; Industrial waste water; Biosensor; Detection limit; Potentiometric method; Bacteria; Hydrolases; Pseudomonadaceae; Carboxamide; Pseudomonas aeruginosa

Acrylamide is a toxic amide with potentially hazardous effects on the environment and human health. This paper reports the results regarding the development of a potentiometric biosensor in order to determine the amount of this amide in wastewater samples. The biosystem consisted of whole cells of P...