Functional properties of sodium channels in cholesterol-depleted K562 cells

• Published in: Cell and tissue biology Vol. 3; no. 5; pp. 461 - 469
• Main Authors: Sudarikova, A. V., Chubinsky-Nadezhdin, V. I., Negulyaev, Yu. A., Morachevskaya, E. A.
• Format: Journal Article
• Language: English
• Published: Dordrecht SP MAIK Nauka/Interperiodica 01.10.2009
• Subjects: Biomedical and Life Sciences; Cell Biology; Life Sciences; actin; methyl-beta-cyclodextrin; sodium channels; plasma membrane; cytoskeleton; cholesterol; cytochalasin; human leukemia cells

In the present paper, functional properties of nonvoltage-gated sodium channels in K562 cells were studied after cholesterol depletion, i.e., under conditions of the destruction of microdomains (rafts). For cholesterol depletion, cells were incubated with methyl-beta-cyclodextrin (MbCD), an oligosac...