The increase in BIK expression following ERK1/2 pathway inhibition is a consequence of G₁ cell-cycle arrest and not a direct effect on BIK protein stability

• Published in: Biochemical journal Vol. 459; no. 3; p. 513
• Main Authors: Sale, Matthew J, Cook, Simon J
• Format: Journal Article
• Language: English
• Published: England 01.05.2014
• Subjects: Antineoplastic Agents - pharmacology; Apoptosis Regulatory Proteins - antagonists & inhibitors; Apoptosis Regulatory Proteins - biosynthesis; Apoptosis Regulatory Proteins - genetics; Apoptosis Regulatory Proteins - metabolism; Bcl-2-Like Protein 11; Cell Line, Tumor; Cyclin-Dependent Kinase 4 - antagonists & inhibitors; Cyclin-Dependent Kinase 4 - metabolism; Cyclin-Dependent Kinase 6 - antagonists & inhibitors; Cyclin-Dependent Kinase 6 - metabolism; G1 Phase - drug effects; Gene Expression Regulation, Neoplastic - drug effects; Humans; MAP Kinase Kinase 1 - antagonists & inhibitors; MAP Kinase Kinase 1 - metabolism; MAP Kinase Kinase 2 - antagonists & inhibitors; MAP Kinase Kinase 2 - metabolism; MAP Kinase Signaling System - drug effects; Membrane Proteins - antagonists & inhibitors; Membrane Proteins - biosynthesis; Membrane Proteins - genetics; Membrane Proteins - metabolism; Mitochondrial Proteins; Neoplasm Proteins - antagonists & inhibitors; Neoplasm Proteins - biosynthesis; Neoplasm Proteins - genetics; Neoplasm Proteins - metabolism; Neoplasms - drug therapy; Neoplasms - enzymology; Neoplasms - metabolism; Proteasome Endopeptidase Complex - chemistry; Proteasome Endopeptidase Complex - metabolism; Proteasome Inhibitors - pharmacology; Protein Kinase Inhibitors - pharmacology; Protein Stability - drug effects; Proteolysis - drug effects; Proto-Oncogene Proteins - antagonists & inhibitors; Proto-Oncogene Proteins - biosynthesis; Proto-Oncogene Proteins - genetics; Proto-Oncogene Proteins - metabolism; RNA Interference; RNA, Small Interfering; Ubiquitination - drug effects

BIK (BCL2-interacting killer) is a pro-apoptotic BH3 (BCL2 homology domain 3)-only protein and a member of the BCL2 protein family. It was proposed recently that BIK abundance is controlled by ERK1/2 (extracellular-signal-regulated kinase 1/2)-catalysed phosphorylation, which targets the protein for...