Shewanella olleyana sp. nov., a marine species isolated from a temperate estuary which produces high levels of polyunsaturated fatty acids

• Published in: International journal of systematic and evolutionary microbiology Vol. 52; no. 6; pp. 2101 - 2106
• Main Authors: Skerratt, J. H, Bowman, J. P, Nichols, P. D
• Format: Journal Article
• Language: English
• Published: Soc General Microbiol 01.11.2002
• ISSN: 1466-5026; 1466-5034; 1466-5034
• DOI: 10.1099/ijs.0.02351-0

J. H. Skerratt, J. P. Bowman and P. D. Nichols Cooperative Research Centre for the Antarctic and Southern Ocean, University of Tasmania, GPO Box 252-80 Hobart, Tasmania, Australia Two polyunsaturated fatty acid (PUFA) producing strains (ACEM 6 and ACEM 9(T)) isolated from a temperate, humic-rich river estuary in Tasmania, Australia, were found to be members of the genus Shewanella. These strains were able to utilize humic compounds (tannic acid) and derivatives (2,6-anthraquinone disulfonate) as sole carbon sources and as electron acceptors for anaerobic respiration. The major fatty acids were typical of the genus Shewanella; however, PUFAs mostly made up of eicosapentaenoic acid were produced at high levels (10.2--23.6% of total fatty acids) and at relatively high incubation temperatures (10.2% at 24 degrees C). Sequence analysis indicated that ACEM 6 and ACEM 9(T) had identical 16S rDNA sequences and were most closely related to Shewanella japonica (sequence similarity 97.1%). DNA hybridization and phenotypic characteristics confirmed that the isolates constituted a novel species of the genus Shewanella, which is designated Shewanella olleyana sp. nov. (type strain ACEM 9(T)=ACAM 644(T)=LMG 21437(T)).