Amplification ofcyclin D1andMDM-2in oesophageal carcinoma
Aims:This study investigated amplification of thecyclin D1andMDM-2genes, and overexpression of the cyclin D1 gene product, in oesophageal carcinoma.Methods:Paired tumour and normal DNA samples from 26 oesophageal adenocarcinomas and 19 squamous cell carcinomas were analysed by Southern blotting with...
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Published in | European journal of surgical oncology Vol. 25; no. 4; pp. 364 - 367 |
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Main Authors | , , , |
Format | Journal Article |
Language | English |
Published |
Elsevier Ltd
01.08.1999
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Subjects | |
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Abstract | Aims:This study investigated amplification of thecyclin D1andMDM-2genes, and overexpression of the cyclin D1 gene product, in oesophageal carcinoma.Methods:Paired tumour and normal DNA samples from 26 oesophageal adenocarcinomas and 19 squamous cell carcinomas were analysed by Southern blotting with specific DNA probes forcyclin D1andMDM-2, and for a control gene (α-lactalbumin). Thecyclin D1andMDM-2gene copy numbers were calculated for each tumour. Expression of thecyclin D1gene was assessed by immunohistochemical analysis of its protein product.Results:Cyclin D1 gene amplification (by a factor of between two and six) was identified in seven tumours (16%).MDM-2gene amplification (by a factor of between two and 11) was identified in 10 tumours (22%). Overexpression of cyclin D1 protein was identified in eight tumours and was significantly associated with gene amplification (P=0.04; Fisher's exact test), and with early T stage (P=0.01; Fisher's exact test).Conclusions:Cyclin D1 andMDM-2amplification and cyclin D1 overexpression occur, although infrequently, in the development of oesophageal carcinoma. Cyclin D1 overexpression may influence tumour behaviour, causing the disease to present at an earlier T stage. The mechanism for this effect is unclear, and warrants further investigation. |
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AbstractList | Aims:This study investigated amplification of thecyclin D1andMDM-2genes, and overexpression of the cyclin D1 gene product, in oesophageal carcinoma.Methods:Paired tumour and normal DNA samples from 26 oesophageal adenocarcinomas and 19 squamous cell carcinomas were analysed by Southern blotting with specific DNA probes forcyclin D1andMDM-2, and for a control gene (α-lactalbumin). Thecyclin D1andMDM-2gene copy numbers were calculated for each tumour. Expression of thecyclin D1gene was assessed by immunohistochemical analysis of its protein product.Results:Cyclin D1 gene amplification (by a factor of between two and six) was identified in seven tumours (16%).MDM-2gene amplification (by a factor of between two and 11) was identified in 10 tumours (22%). Overexpression of cyclin D1 protein was identified in eight tumours and was significantly associated with gene amplification (P=0.04; Fisher's exact test), and with early T stage (P=0.01; Fisher's exact test).Conclusions:Cyclin D1 andMDM-2amplification and cyclin D1 overexpression occur, although infrequently, in the development of oesophageal carcinoma. Cyclin D1 overexpression may influence tumour behaviour, causing the disease to present at an earlier T stage. The mechanism for this effect is unclear, and warrants further investigation. |
Author | Hardwick, R.H. Morgan, R.J. Newcomb, P.V. Alderson, D. |
Author_xml | – sequence: 1 givenname: R.J. surname: Morgan fullname: Morgan, R.J. – sequence: 2 givenname: P.V. surname: Newcomb fullname: Newcomb, P.V. – sequence: 3 givenname: R.H. surname: Hardwick fullname: Hardwick, R.H. – sequence: 4 givenname: D. surname: Alderson fullname: Alderson, D. |
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Title | Amplification ofcyclin D1andMDM-2in oesophageal carcinoma |
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