Lysing Yeast Cells for Immunoprecipitation Using a Coffee Grinder

• Published in: Cold Spring Harbor protocols Vol. 2020; no. 11
• Main Authors: DeCaprio, James, Kohl, Thomas O
• Format: Journal Article
• Language: English
• Published: United States 01.11.2020

This protocol describes the freezing of yeast in liquid nitrogen (LN ) to form small "beans" that can be ground using a simple propeller-blade coffee grinder. The method is ideally suited for lysate preparations from larger yeast cultures ranging from 50 mL to 5 L and displays the advantag...