Hydrolytic properties of phenylalanyl- and N-acetylphenylalanyl adenylate anhydrides

• Published in: Origins of life and evolution of biospheres Vol. 15; no. 1; p. 45
• Main Authors: Lacey, Jr, J C, Senaratne, N, Mullins, Jr, D W
• Format: Journal Article
• Language: English
• Published: Netherlands 01.03.1984
• Subjects: Adenosine Monophosphate - analogs & derivatives; Adenosine Monophosphate - chemistry; Carbonates - chemistry; Catalysis; Evolution, Molecular; Hydrogen-Ion Concentration; Hydrolysis; Kinetics; Peptide Biosynthesis; Proteins - chemical synthesis

We have used a novel spectrophotometric method to study the hydrolysis of N-acetylphenylalanyl adenylate anhydride (AcPhe-AMP) and phenylalanyl-adenylate anhydride (Phe-AMP) at low concentrations (10(-5) M), 25 degrees C, constant buffer concentration (0.05 M), and as a function of pH. While Phe-AMP...