Expression and properties of diacylglycerol acyltransferase from cell-suspension cultures of oilseed rape

• Published in: Biochemical Society transactions Vol. 28; no. 6; p. 684
• Main Authors: Weselake, R J, Nykiforuk, C L, Laroche, A, Patterson, N A, Wiehler, W B, Szarka, S J, Moloney, M M, Tari, L W, Derekh, U
• Format: Journal Article
• Language: English
• Published: England 01.12.2000
• Subjects: Acyl Coenzyme A - metabolism; Acyltransferases - genetics; Acyltransferases - metabolism; Brassica - cytology; Brassica - enzymology; Cells, Cultured; Diacylglycerol O-Acyltransferase; Kinetics; Protein Biosynthesis; Recombinant Proteins - metabolism; Substrate Specificity; Time Factors; Transcription, Genetic

The expression of diacylglycerol acyltransferase (DGAT, EC 2.3.1.20) with predicted molecular mass of 56.9. kDa (BnDGAT1) was examined using microspore-derived cell suspension cultures of oilseed rape (Brassica napus L. cv Jet Neuf). As well, a recombinant histidine-tagged N-terminal fragment of BnD...