Hydrogel-supported poly(L-lactic acid) and polystyrene microsphere-based three-dimensional culture systems for in vitro cell expansion
The in vitro expansion of stem cells is important for their application in different life science fields such as cellular tissue and organ repair. An objective of this paper was to achieve static cell culture in vitro through peptide hydrogel-supported microspheres (MSs). The peptides, with their ge...
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| Published in | Frontiers of materials science Vol. 18; no. 2 |
|---|---|
| Main Authors | , , , |
| Format | Journal Article |
| Language | English |
| Published |
Beijing
Higher Education Press
01.06.2024
Springer Nature B.V |
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| ISSN | 2095-025X 2095-0268 |
| DOI | 10.1007/s11706-024-0682-z |
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| Abstract | The
in vitro
expansion of stem cells is important for their application in different life science fields such as cellular tissue and organ repair. An objective of this paper was to achieve static cell culture
in vitro
through peptide hydrogel-supported microspheres (MSs). The peptides, with their gel-forming properties, microstructures, and mechanical strengths characterized, were found to have good support for the MSs and to be injectable. The internal structures of poly(L-lactic acid) microspheres (PLLA-MSs) and polystyrene microspheres (PS-MSs) made in the laboratory were observed and statistically analyzed in terms of particle size and pore size, following which the co-cultured MSs with cells were found to have good cell adhesion. In addition, three-dimensional (3D) culturing of cells was performed on the peptide and microcarrier composite scaffolds to measure cell viability and cell proliferation. The results showed that the peptides could be stimulated by the culture medium to self-assembly form a 3D fiber network structure. Under the peptide-MS composite scaffold-based cell culture system, further enhancement of the cell culture effect was measured. The peptide-MS composite scaffolds have great potential for the application in 3D cell culture and
in vitro
cell expansion. |
|---|---|
| AbstractList | The
in vitro
expansion of stem cells is important for their application in different life science fields such as cellular tissue and organ repair. An objective of this paper was to achieve static cell culture
in vitro
through peptide hydrogel-supported microspheres (MSs). The peptides, with their gel-forming properties, microstructures, and mechanical strengths characterized, were found to have good support for the MSs and to be injectable. The internal structures of poly(L-lactic acid) microspheres (PLLA-MSs) and polystyrene microspheres (PS-MSs) made in the laboratory were observed and statistically analyzed in terms of particle size and pore size, following which the co-cultured MSs with cells were found to have good cell adhesion. In addition, three-dimensional (3D) culturing of cells was performed on the peptide and microcarrier composite scaffolds to measure cell viability and cell proliferation. The results showed that the peptides could be stimulated by the culture medium to self-assembly form a 3D fiber network structure. Under the peptide-MS composite scaffold-based cell culture system, further enhancement of the cell culture effect was measured. The peptide-MS composite scaffolds have great potential for the application in 3D cell culture and
in vitro
cell expansion. The in vitro expansion of stem cells is important for their application in different life science fields such as cellular tissue and organ repair. An objective of this paper was to achieve static cell culture in vitro through peptide hydrogel-supported microspheres (MSs). The peptides, with their gel-forming properties, microstructures, and mechanical strengths characterized, were found to have good support for the MSs and to be injectable. The internal structures of poly(L-lactic acid) microspheres (PLLA-MSs) and polystyrene microspheres (PS-MSs) made in the laboratory were observed and statistically analyzed in terms of particle size and pore size, following which the co-cultured MSs with cells were found to have good cell adhesion. In addition, three-dimensional (3D) culturing of cells was performed on the peptide and microcarrier composite scaffolds to measure cell viability and cell proliferation. The results showed that the peptides could be stimulated by the culture medium to self-assembly form a 3D fiber network structure. Under the peptide-MS composite scaffold-based cell culture system, further enhancement of the cell culture effect was measured. The peptide-MS composite scaffolds have great potential for the application in 3D cell culture and in vitro cell expansion. |
| ArticleNumber | 240682 |
| Author | Chen, Jiaxuan Hao, Huaying Sun, Lihong Liang, Jun |
| Author_xml | – sequence: 1 givenname: Huaying surname: Hao fullname: Hao, Huaying organization: College of Light Industry Science and Engineering, Tianjin University of Science & Technology – sequence: 2 givenname: Lihong surname: Sun fullname: Sun, Lihong organization: College of Food Science and Engineering, Tianjin University of Science & Technology – sequence: 3 givenname: Jiaxuan surname: Chen fullname: Chen, Jiaxuan organization: College of Light Industry Science and Engineering, Tianjin University of Science & Technology – sequence: 4 givenname: Jun surname: Liang fullname: Liang, Jun email: jliang1118@yeah.net organization: State Key Laboratory of Food Nutrition and Safety, Tianjin University of Science & Technology, College of Light Industry Science and Engineering, Tianjin University of Science & Technology |
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in vitro
expansion of stem cells is important for their application in different life science fields such as cellular tissue and organ repair. An objective... The in vitro expansion of stem cells is important for their application in different life science fields such as cellular tissue and organ repair. An objective... |
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| SubjectTerms | Cell adhesion Cell culture Chemistry and Materials Science Hydrogels Materials Science Microspheres Peptides Polylactic acid Polystyrene resins Pore size Research Article Scaffolds Self-assembly Stem cells |
| Title | Hydrogel-supported poly(L-lactic acid) and polystyrene microsphere-based three-dimensional culture systems for in vitro cell expansion |
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