Infection of animal coronaviruses into a bat cell line from the kidney of Pipistrellus abramus

Although most coronaviruses are thought to have their origins in bats, there is little information on whether bats are still susceptible to the currently prevalent coronaviruses. We generated a continuous cell line from a kidney of Pipistrellus abramus (P. abramus) by serial passaging of the cells,...

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Published inJournal of Veterinary Medical Science p. 24-0406
Main Authors NAKAGAWA, Keisuke, MIYAWAKI, Shingo, MORIBE, Junji, MASATANI, Tatsunori, SAITO, Taizo, TAKASHIMA, Yasuhiro
Format Journal Article
LanguageEnglish
Published Japan JAPANESE SOCIETY OF VETERINARY SCIENCE 11.07.2025
Subjects
bovine coronavirus
Pipistrellus abramus
porcine epidemic diarrhea virus
virus entry
virus infection
virus entry
porcine epidemic diarrhea virus
Pipistrellus abramus
bovine coronavirus
virus infection
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Abstract Although most coronaviruses are thought to have their origins in bats, there is little information on whether bats are still susceptible to the currently prevalent coronaviruses. We generated a continuous cell line from a kidney of Pipistrellus abramus (P. abramus) by serial passaging of the cells, which could be proliferated for more than 80 passages without any immortalization process. We found that porcine epidemic diarrhea virus (PEDV) and bovine coronavirus (BCoV) were able to infect and generate progeny viruses in P. abramus-derived cells in the presence of trypsin, although the replication efficiency was quite low. The findings suggest that P. abramus could be susceptible to PEDV and BCoV and may play a potential role as a transmitter of the viruses.
AbstractList Although most coronaviruses are thought to have their origins in bats, there is little information on whether bats are still susceptible to the currently prevalent coronaviruses. We generated a continuous cell line from a kidney of Pipistrellus abramus (P. abramus) by serial passaging of the cells, which could be proliferated for more than 80 passages without any immortalization process. We found that porcine epidemic diarrhea virus (PEDV) and bovine coronavirus (BCoV) were able to infect and generate progeny viruses in P. abramus-derived cells in the presence of trypsin, although the replication efficiency was quite low. The findings suggest that P. abramus could be susceptible to PEDV and BCoV and may play a potential role as a transmitter of the viruses.Although most coronaviruses are thought to have their origins in bats, there is little information on whether bats are still susceptible to the currently prevalent coronaviruses. We generated a continuous cell line from a kidney of Pipistrellus abramus (P. abramus) by serial passaging of the cells, which could be proliferated for more than 80 passages without any immortalization process. We found that porcine epidemic diarrhea virus (PEDV) and bovine coronavirus (BCoV) were able to infect and generate progeny viruses in P. abramus-derived cells in the presence of trypsin, although the replication efficiency was quite low. The findings suggest that P. abramus could be susceptible to PEDV and BCoV and may play a potential role as a transmitter of the viruses.
Although most coronaviruses are thought to have their origins in bats, there is little information on whether bats are still susceptible to the currently prevalent coronaviruses. We generated a continuous cell line from a kidney of Pipistrellus abramus (P. abramus) by serial passaging of the cells, which could be proliferated for more than 80 passages without any immortalization process. We found that porcine epidemic diarrhea virus (PEDV) and bovine coronavirus (BCoV) were able to infect and generate progeny viruses in P. abramus-derived cells in the presence of trypsin, although the replication efficiency was quite low. The findings suggest that P. abramus could be susceptible to PEDV and BCoV and may play a potential role as a transmitter of the viruses.
ArticleNumber 24-0406
Author SAITO, Taizo
MORIBE, Junji
MASATANI, Tatsunori
MIYAWAKI, Shingo
TAKASHIMA, Yasuhiro
NAKAGAWA, Keisuke
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  organization: Faculty of Applied Biological Science, Joint Department of Veterinary Medicine, Gifu University
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  organization: Faculty of Applied Biological Science, Joint Department of Veterinary Medicine, Gifu University
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  fullname: TAKASHIMA, Yasuhiro
  organization: Faculty of Applied Biological Science, Joint Department of Veterinary Medicine, Gifu University
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Keywords virus entry
porcine epidemic diarrhea virus
Pipistrellus abramus
bovine coronavirus
virus infection
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SubjectTerms bovine coronavirus
Pipistrellus abramus
porcine epidemic diarrhea virus
virus entry
virus infection
Title Infection of animal coronaviruses into a bat cell line from the kidney of Pipistrellus abramus
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