Radioimmunoassay of morphine and morphine-like substances in biological fluids and human tissues

A radioimmunoassay is described for the determination of morphine and morphine-like substances in plasma, serum, biological fluids and tissue homogenates using an antiserum to morphine-6-hemisuccinyl-BSA and 125I-morphine as the labelled tracer. In the B/F separation with ammonium sulphate, calcium...

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Bibliographic Details
Published inRicerca in clinica e in laboratorio Vol. 7; no. 2; p. 179
Main Authors Lafisca, S, Bolelli, G, Mosca, R, Zanon, C
Format Journal Article
LanguageEnglish
Published Italy 01.04.1977
Subjects
Animals
Codeine - analysis
Humans
Immune Sera
Iodine Radioisotopes
Meperidine - analysis
Methadone - analysis
Morphine - analysis
Morphine - immunology
Morphine Dependence - diagnosis
Nalorphine - analysis
Rabbits - immunology
Radioimmunoassay - methods
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Summary:A radioimmunoassay is described for the determination of morphine and morphine-like substances in plasma, serum, biological fluids and tissue homogenates using an antiserum to morphine-6-hemisuccinyl-BSA and 125I-morphine as the labelled tracer. In the B/F separation with ammonium sulphate, calcium sulphate was added to make the precipitate more compact. Some parameters related to the use of this method in direct assay on plasma, serum, other biological fluids and tissue homogenates were evaluated.
ISSN:0390-5748
DOI:10.1007/BF02879482