Assessment of GC-MS in Detecting Changes in the Levels of Metabolites Using a Spike-in Experiment in Human Plasma

• Published in: Metabolomics (Los Angeles, CA) Vol. 6; no. 2
• Main Authors: Varghese, Rency S, Poto, Cristina Di, Tu, Chao, Ranjbar, Mohammad R Nezami, Luo, Yue, Choi, Jiwon, Tadesse, Mahlet G, Ressom, Habtom W
• Format: Journal Article
• Language: English
• Published: United States 2016
• Subjects: GC-MS; Metabolomics; Internal standards; Latin square design; Spike-in experiment

Gas Chromatography coupled with Mass Spectrometry (GC-MS) has been broadly used for the detection of changes in metabolite levels in complex samples. Internal Standards (IS) spiked into a complex background at different concentrations help assess the capability of GC-MS in detecting changes in metab...