The Expression of VacA in BCF of Helicobacter Pylori and Its Relationship to Vacuolated Effect

• Published in: Current medical science Vol. 22; no. 2; pp. 100 - 102
• Main Authors: Li, Shi, Xiaohua, Hou, Cuiqiong, Yi, Jinkun, Zhang
• Format: Journal Article
• Language: English
• Published: China Infective Disease Center, Guangzhou 510602%Department of Internal Medicine, Xiehe Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022 2002
• Subjects: Adolescent; Adult; Bacterial Proteins - genetics; Female; Genotype; Helicobacter; Helicobacter Infections - microbiology; Helicobacter pylori - genetics; Humans; Male; Middle Aged; Peptic Ulcer - microbiology; pylori; SDS-PAGE; Stomach Diseases - microbiology; VacA; Vacuoles; Helicobacter pylori; VacA SDS-PAGE
• ISSN: 1672-0733; 2096-5230; 1993-1352; 2523-899X
• DOI: 10.1007/BF02857664

The vacuolated effect of Helicobacter (H. Pylori) and its relationship to vacuolated cyto toxin antigen (VacA) were investigated by the method of cytotoxic test and SDS-pobyacrylamide gel electrophoresis (SDS-PAGE). Of the 62 clinical isolates, the broth culture filter (BCF) of 43 strains causecl the Vero cell intracytoplasmically vacuolated. H. Pylori strains were divided into H. Pylori (Toxin+) group with vacuolated effect and H. Pylori (Toxin-) group without vacuolated effect. The analysis of the BCF of H. Pylori (Toxin+) and that of H. Pylori (Toxin-) was studied by SDS-PAGE and Scan reader. A kind of protein with 87 ku molecular weight was recognized in the BCF of 30.23 % (13/43) H. Pylori (Toxin+) strains but in none of that of H. Pylori (Toxin-) strains, the difference was statistically significant (P＜0. 05). There was a significant and concordant relation ship between OD of the protein band with 87 ku molecular weight and titer of vacuolated activity of H. Pylori(Toxin+) (r=0. 67 and P＜0. 05 by linear regression analysis). H. Pylori strains were di-vided into H. Pylori (Toxin+) group with vacuolated effect and H. Pylori (Toxin-) group without vacuolated effect. The vacuolated effect of H. Pylori (Toxin+) was caused by the protein with 87 ku molecular weight (VacA).