Morphologic study on frozen storage of a hamster malignant fibrous histiocytoma induced by DMBA heterotransplanted in nude mice

• Published in: Japanese Journal of Oral and Maxillofacial Surgery Vol. 36; no. 3; pp. 514 - 523
• Main Authors: TSUBOI, Kengo, HONDA, Takeshi, HAYASHI, Noboru, KOMOTO, Katsuma, SATOH, Toshimasa
• Format: Journal Article
• Language: English
• Published: Japanese Society of Oral and Maxillofacial Surgeons 1990
• Subjects: frozen storage; malignant fibrous histiocytoma; X-ray microanalysis
• ISSN: 0021-5163; 2186-1579
• DOI: 10.5794/jjoms.36.514

Hamster MFH cells induced by DMBA heterotransplanted in nude mice were frozen and kept at -80°C. Morphologic changes of the cells after frozen preservation were investigated and the distribution of organizing elements of undifferentiated cells overlapping the cellular ultrastructure was analysed as a fundamental study. The results obtained were as follows: 1. Light microscopic findings: After frozen preservation, the opulation of fibroblast-like cells decreased and the tumor was composed mainly of undifferentiated, multinucleated, giant cells. 2. Electron microscopic findings: After frozen preservation, fibroblast-like cell changed rough endoplasmic reticulum frozen dilated cisternae to narrow. Multinucleated giant cells in histiocyte-like cell type increased. 3. A large amount of sodium, phosphorus, sulfur and potassium in erythrocytes, marginal heterochromatin, and nucleoli in undifferentiated mesenchymal cells were found by X-ray microanalysis. But there was a difference in weight percent of these elements between the three tissues.