Interception of ‘Candidatus Phytoplasma solani’ in Brazil in apple tree propagative material imported from France
Apple plants from France introduced in Brazil for research purposes were subjected to a phytosanitary analysis at the Plant Quarantine Laboratory of Embrapa Genetic Resources and Biotechnology (Cenargen). After grafting onto healthy apple rootstock, some plants showed phytoplasma-infection symptoms....
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| Published in | Arquivos do Instituto Biológico (São Paulo) Vol. 84 |
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| Main Authors | , , , , |
| Format | Journal Article |
| Language | English |
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Instituto Biológico
01.02.2018
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| Abstract | Apple plants from France introduced in Brazil for research purposes were subjected to a phytosanitary analysis at the Plant Quarantine Laboratory of Embrapa Genetic Resources and Biotechnology (Cenargen). After grafting onto healthy apple rootstock, some plants showed phytoplasma-infection symptoms. Polymerase Chain Reaction (PCR) tests yielded DNA fragments of the expected size for phytoplasmas. DNA sequencing revealed an identity of the 16S rDNA nucleotide sequence of 98-99% with ‘Candidatus Phytoplasma solani’. This phytoplasma species is responsible for losses in European apple orchards and has not been reported in Brazil. According to the Federal Legislation on Plant Protection, the plants were incinerated to avoid the introduction of this exotic pest in Brazil.
RESUMO: Plantas de macieira originárias da França introduzidas no Brasil para fins de pesquisa foram submetidas à análise no Laboratório de Quarentena Vegetal da Embrapa Recursos Genéticos e Biotecnologia (Cenargen). Após realizar a enxertia em porta-enxertos sadios, algumas plantas apresentaram sintomas típicos de infecção por fitoplasmas. Os testes por meio da técnica de reação em cadeia da polimerase (PCR) obtiveram fragmentos de DNA do tamanho esperado para fitoplasmas. O sequenciamento de DNA revelou uma identidade de 98-99% da região 16 rDNA dos fitoplasmas encontrados com o fitoplasma ‘Candidatus Phytoplasma solani’. Essa espécie de fitoplasma é responsável por perdas em pomares de maçã europeus e não foi relatada no Brasil. De acordo com a Legislação Federal de Proteção de Plantas, as plantas de macieira foram incineradas para evitar a entrada dessa praga exótica no Brasil. |
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| AbstractList | Apple plants from France introduced in Brazil for research purposes were subjected to a phytosanitary analysis at the Plant Quarantine Laboratory of Embrapa Genetic Resources and Biotechnology (Cenargen). After grafting onto healthy apple rootstock, some plants showed phytoplasma-infection symptoms. Polymerase Chain Reaction (PCR) tests yielded DNA fragments of the expected size for phytoplasmas. DNA sequencing revealed an identity of the 16S rDNA nucleotide sequence of 98-99% with ‘Candidatus Phytoplasma solani’. This phytoplasma species is responsible for losses in European apple orchards and has not been reported in Brazil. According to the Federal Legislation on Plant Protection, the plants were incinerated to avoid the introduction of this exotic pest in Brazil.
RESUMO: Plantas de macieira originárias da França introduzidas no Brasil para fins de pesquisa foram submetidas à análise no Laboratório de Quarentena Vegetal da Embrapa Recursos Genéticos e Biotecnologia (Cenargen). Após realizar a enxertia em porta-enxertos sadios, algumas plantas apresentaram sintomas típicos de infecção por fitoplasmas. Os testes por meio da técnica de reação em cadeia da polimerase (PCR) obtiveram fragmentos de DNA do tamanho esperado para fitoplasmas. O sequenciamento de DNA revelou uma identidade de 98-99% da região 16 rDNA dos fitoplasmas encontrados com o fitoplasma ‘Candidatus Phytoplasma solani’. Essa espécie de fitoplasma é responsável por perdas em pomares de maçã europeus e não foi relatada no Brasil. De acordo com a Legislação Federal de Proteção de Plantas, as plantas de macieira foram incineradas para evitar a entrada dessa praga exótica no Brasil. Apple plants from France introduced in Brazil for research purposes were subjected to a phytosanitary analysis at the Plant Quarantine Laboratory of Embrapa Genetic Resources and Biotechnology (Cenargen). After grafting onto healthy apple rootstock, some plants showed phytoplasma-infection symptoms. Polymerase Chain Reaction (PCR) tests yielded DNA fragments of the expected size for phytoplasmas. DNA sequencing revealed an identity of the 16S rDNA nucleotide sequence of 98-99% with ‘Candidatus Phytoplasma solani’. This phytoplasma species is responsible for losses in European apple orchards and has not been reported in Brazil. According to the Federal Legislation on Plant Protection, the plants were incinerated to avoid the introduction of this exotic pest in Brazil. |
| Author | Sanches, Márcio Martinello Eckstein, Barbara Botelho, Stephanie Regina Alves Fernandes, Fernanda Rausch Oliveira, Mayara Luana Coelho Alves de |
| Author_xml | – sequence: 1 givenname: Barbara surname: Eckstein fullname: Eckstein, Barbara organization: Embrapa Recursos Genéticos e Biotecnologia, Brazil – sequence: 2 givenname: Stephanie Regina Alves surname: Botelho fullname: Botelho, Stephanie Regina Alves organization: Embrapa Recursos Genéticos e Biotecnologia, Brazil – sequence: 3 givenname: Mayara Luana Coelho Alves de surname: Oliveira fullname: Oliveira, Mayara Luana Coelho Alves de organization: Embrapa Recursos Genéticos e Biotecnologia, Brazil – sequence: 4 givenname: Fernanda Rausch surname: Fernandes fullname: Fernandes, Fernanda Rausch organization: Embrapa Recursos Genéticos e Biotecnologia, Brazil – sequence: 5 givenname: Márcio Martinello surname: Sanches fullname: Sanches, Márcio Martinello organization: Embrapa Recursos Genéticos e Biotecnologia, Brazil |
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| Cites_doi | 10.1099/ijs.0.044750-0 10.4236/ajps.2014.512191 10.1094/PDIS-01-14-0026-PDN 10.1099/00207713-49-3-1275 10.1093/molbev/mst197 10.1099/00207713-48-4-1153 10.1099/ijs.0.63935-0 10.1094/PDIS-93-8-0789 10.1111/j.1439-0434.2009.01586.x 10.1099/ijs.0.02854-0 10.1590/S0100-54052007000100004 |
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| References_xml | – volume: 63 start-page: 2879 year: 2013 ident: ref11 article-title: ‘Candidatus Phytoplasma solani’, a novel taxon associated with stolbur and bois noir-related diseases of plants publication-title: International Journal of Systematic and Evolutionary Microbiology doi: 10.1099/ijs.0.044750-0 contributor: fullname: QUAGLINO F. – volume: 5 start-page: 1763 issue: 12 year: 2014 ident: ref1 article-title: Phytoplasmas and Phytoplasmas Diseases: A Severe Threat to Agriculture publication-title: American Journal of Plant Sciences doi: 10.4236/ajps.2014.512191 contributor: fullname: BERTACCINI A. – volume: 64 start-page: S97 year: 2011 ident: ref9 article-title: Molecular detection and identification of group 16SrV and 16SrXII phytoplasmas associated with potatoes in Colombia publication-title: Bulletin of Insectology contributor: fullname: MEJIA J.F. – volume: 98 start-page: 1151 issue: 8 year: 2014 ident: ref10 article-title: First report of ‘Candidatus Phytoplasma solani’ and ‘Ca. P. convolvuli’ associated with grapevine Bois Noir and bindweed yellows, respectively, in Georgia publication-title: Plant Disease doi: 10.1094/PDIS-01-14-0026-PDN contributor: fullname: QUAGLINO F. – volume: 54 start-page: 1 issue: 54 year: 2013 ident: ref2 article-title: Cenário da cadeia produtiva da maçã publication-title: Informativo – volume: 49 start-page: 1275 issue: 3 year: 1999 ident: ref13 article-title: ‘Candidatus Phytoplasma japonicum’, a new phytoplasma taxon associated with Japanese Hydrangea phyllody publication-title: International Journal of Systematic Bacteriology doi: 10.1099/00207713-49-3-1275 contributor: fullname: SAWAYANAGI T. – volume: 30 start-page: 2725 issue: 12 year: 2013 ident: ref14 article-title: MEGA6: Molecular Evolutionary Genetics Analysis version 6.0 publication-title: Molecular Biology and Evolution doi: 10.1093/molbev/mst197 contributor: fullname: TAMURA K. – start-page: 1 volume-title: Introduction year: 2006 ident: ref16 contributor: fullname: YADAV P.R. – year: 2003 ident: ref8 contributor: fullname: MARINHO V.L.A. – volume: 48 start-page: 1153 year: 1998 ident: ref7 article-title: Revised classification scheme of phytoplasmas based on RFLP analyses of 16S rRNA ribosomal protein gene sequences publication-title: International Journal of Systematic Bacteriology doi: 10.1099/00207713-48-4-1153 contributor: fullname: LEE I-M. – volume: 56 start-page: 277 issue: 1 year: 2006 ident: ref15 article-title: ‘Candidatus Phytoplasma fragariae’, a novel phytoplasma taxon discovered in yellows diseased strawberry, Fragaria x Ananassa publication-title: International Journal of Systematic and Evolutionary Microbiology doi: 10.1099/ijs.0.63935-0 contributor: fullname: VALIUNAS D. – volume: 93 start-page: 789 issue: 8 year: 2009 ident: ref5 article-title: Phytoplasmas associated with grapevine yellows disease in Chile publication-title: Plant Disease doi: 10.1094/PDIS-93-8-0789 contributor: fullname: GAJARDO A. – volume: 47 start-page: 262 issue: 2 year: 1997 ident: ref3 article-title: “Candidatus Phytoplasma australiense”, a new phytoplasma taxon associated with Australian grapevine yellows publication-title: International Journal of Systematic and Evolutionary Microbiology contributor: fullname: DAVIS R.E. – volume: 158 start-page: 100 issue: 2 year: 2010 ident: ref4 article-title: Occurrence of phytoplasmas related to stolbur and to ‘Candidatus Phytoplasma japonicum’ in woody host plants in China publication-title: Journal of Phytopathology doi: 10.1111/j.1439-0434.2009.01586.x contributor: fullname: DUDUK B. – volume: 54 start-page: 1243 issue: 4 year: 2004 ident: ref6 article-title: ‘Candidatus Phytoplasma’, a taxon for the wall-less, non helical prokaryotes that colonize plant phloem and insects publication-title: International Journal of Systematic Microbiology doi: 10.1099/ijs.0.02854-0 – volume: 33 start-page: 30 issue: 1 year: 2007 ident: ref12 article-title: Evidência molecular da ocorrência de um fitoplasma associado ao lenho mole da macieira publication-title: Summa Phytopathologica doi: 10.1590/S0100-54052007000100004 contributor: fullname: RIBEIRO L.F.C. |
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