Detection of Telomerase Activity and the Expression of Telomerase Subunits in the Patients with Acute Myelogenous Leukaemia

• Published in: Current medical science Vol. 24; no. 1; pp. 48 - 51
• Main Author: 李一荣 吴健民 王琳 陈凤花 胡丽华
• Format: Journal Article
• Language: English
• Published: China Department of Laboratory ＆ Institute of Immunology, Union Hospital, Tongji Medical College, Huazhong University of Science and Techonology , Wuhan 430022%Li f e Scientific College, Wuhan University, Wuhan 450072%Trans fusion Department, Union Hospital, Tongji Medical College, Huazhong University of Science and Techonology ,Wuhan 430022 2004
• Subjects: Carrier Proteins - metabolism; Child; DNA-Binding Proteins; ELISA; Humans; Leukemia, Myeloid, Acute - enzymology; Reverse Transcriptase Polymerase Chain Reaction; RNA - metabolism; RNA, Messenger - metabolism; RNA-Binding Proteins; Telomerase - metabolism; 急性髓性白血病; 核蛋白聚合酶; 端粒酶亚型; 端粒酶活性; 聚合酶链反应; telomerase RNA component; telomerase; telomerase reverse transcriptase; telomerase associated protein 1; acute myelogenous leukaemia
• DOI: 10.1007/BF02830704
• ISSN: 1672-0733; 2096-5230

Telomerase activity and the expression of telomerase subunits (for example, telomerase reverse transcriptase and telomerase associated protein 1 and telomerase RNA component) of peripheral white blood cells were detected in the patients with acute myelogenous leukaemia (AML) and the correlation between telomerase activity and the expression of telomerase subunits was observed. In 94 peripheral white blood cells from 18 healthy volunteers and 76 patients with AML,including 31 AML at initial presentation, 24 at relapse and 21 at complete remission, the telomerase activity and telomerase subunits mRNA or RNA were detected by PCR-ELISA and RT-PCR respectively. The results showed that the positive rate of telomerase from patients with AML at initial presentation, at relapse and at complete remission was 74.1%, 79.2% and 4.8% respectively.The positive rate of telomerase reverse transcriptase mRNA from healthy volunteers, AML at initial presentation, AML at relapse and AML at complete remission was 5.6%, 80.6%, 83.3%and 9.5% respectively. The positive rate of telomerase associated protein 1 mRNA and telomerase RNA component in all samples were 100%. It was suggested that the up-regulation of telomerase activity and the expression of telomerase reverse transcriptase is correlated closely with the occurrence and relapse of AML, so telomerase activity and the expression of telomerase reverse transcriptase may be used to estimate the curative effect and predict relapse of AML. Moreover, the upregulation of telomerase activity is correlated with the expression of telomerase reverse transcriptase significantly.