Real-time PCR assay for detection of Environmental DNA from Chironex yamaguchii
The box jellyfish Chironex yamaguchii is a highly venomous cubomedusa, also known as “habu-kurage” in Japan. It is a significant threat to marine activities in Okinawa in southern Japan. Annually, envenomation of the species causes nearly 100 to 200 injuries in the Okinawa Prefecture. The present st...
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| Published in | Medical Entomology and Zoology Vol. 74; no. 1; pp. 13 - 20 |
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| Main Authors | , , , , , , , , |
| Format | Journal Article |
| Language | English Japanese |
| Published |
Tokyo
The Japan Society of Medical Entomology and Zoology
25.03.2023
Japan Science and Technology Agency |
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| Online Access | Get full text |
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| Abstract | The box jellyfish Chironex yamaguchii is a highly venomous cubomedusa, also known as “habu-kurage” in Japan. It is a significant threat to marine activities in Okinawa in southern Japan. Annually, envenomation of the species causes nearly 100 to 200 injuries in the Okinawa Prefecture. The present study developed a species-specific real-time PCR assay to analyze environmental DNA (eDNA) for rapid detection of jellyfish in water samples. Primers and probe were designed based on the sequences of the mitochondrial cytochrome c oxidase subunit I region using tissue samples of nine specimens obtained from the Okinawa Prefecture. The specificity of the real-time PCR assay was confirmed by amplifying the DNA samples of 10 different box jellyfish species. A practical assay for eDNA detection was successful on water samples obtained from the natural habitat of C. yamaguchii. In addition, the degradation rate of eDNA in the tank experiment was 65% of the initial concentration at 1 h, 22% at 12 h, and 3% at 48 h. |
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| AbstractList | The box jellyfish Chironex yamaguchii is a highly venomous cubomedusa, also known as “habu-kurage” in Japan. It is a significant threat to marine activities in Okinawa in southern Japan. Annually, envenomation of the species causes nearly 100 to 200 injuries in the Okinawa Prefecture. The present study developed a species-specific real-time PCR assay to analyze environmental DNA (eDNA) for rapid detection of jellyfish in water samples. Primers and probe were designed based on the sequences of the mitochondrial cytochrome c oxidase subunit I region using tissue samples of nine specimens obtained from the Okinawa Prefecture. The specificity of the real-time PCR assay was confirmed by amplifying the DNA samples of 10 different box jellyfish species. A practical assay for eDNA detection was successful on water samples obtained from the natural habitat of C. yamaguchii. In addition, the degradation rate of eDNA in the tank experiment was 65% of the initial concentration at 1 h, 22% at 12 h, and 3% at 48 h. |
| ArticleNumber | 2213 |
| Author | Toshino, Sho Hanahara, Nozomi Kuba, Yumani Fukuchi, Yoshimune Oka, Shin-ichiro Teruya, Morimi Tanimoto, Miyako Azama, Yasuhito Miyagi, Ayano |
| Author_xml | – sequence: 1 fullname: Azama, Yasuhito organization: Okinawa Prefectural Institute of Health and Environment – sequence: 2 fullname: Oka, Shin-ichiro organization: Okinawa Churashima Foundation – sequence: 3 fullname: Teruya, Morimi organization: Okinawa Prefectural Institute of Health and Environment – sequence: 4 fullname: Toshino, Sho organization: Kuroshio Biological Research Foundation – sequence: 5 fullname: Tanimoto, Miyako organization: Okinawa Churashima Foundation – sequence: 6 fullname: Hanahara, Nozomi organization: Okinawa Churashima Foundation – sequence: 7 fullname: Kuba, Yumani organization: Okinawa Prefectural Institute of Health and Environment – sequence: 8 fullname: Miyagi, Ayano organization: Okinawa Prefectural Institute of Health and Environment – sequence: 9 fullname: Fukuchi, Yoshimune organization: Miyako Regional Public Health Center |
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| Cites_doi | 10.1093/molbev/msy096 10.3800/pbr.14.320 10.1093/nar/gkm306 10.3389/fmars.2021.640527 10.3390/ijerph18010219 10.1002/ams2.469 10.1002/edn3.121 10.1002/ece3.4802 10.1002/edn3.132 10.1016/j.toxicon.2009.02.006 10.1007/s12562-018-1282-6 10.1371/journal.pone.0173073 10.1271/kagakutoseibutsu.57.380 10.2336/nishinihonhifu.74.519 10.11646/zootaxa.2030.1.5 10.1002/edn3.181 10.2112/SI78-014.1 |
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| Copyright | 2023 The Japan Society of Medical Entomology and Zoology Copyright Japan Science and Technology Agency 2023 |
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| References | Jo, T., Murakami, H., Yamamoto, S., Masuda, R. and Minamoto, T. 2019. Effect of water temperature and fish biomass on environmental DNA shedding, degradation, and size distribution. Ecol. Evol., 9: 1135–1146. Kawamura, M., Ueno, S., Iwanaga, S., Oshiro, N. and Kubota, S. 2003. The relationship between fine rings in the statolith and growth of the cubomedusa Chiropsalmus quadrigatus (Cnidaria: Cubozoa) from Okinawa Island, Japan. Plankton Biol. Ecol., 50: 37–42. 沖縄環境保全研究所．2008．平成19年度ハブクラゲ分布調査及び採集・輸送業務報告書，154 pp. 岩永節子，金本昭彦．2004．ハブクラゲ等海洋有毒生物の生態と防除に関する調査研究．平成15年度 亜熱帯地域の有害・有毒生物に関する調査研究報告書，pp.57–83. 財団法人 亜熱帯総合研究所，沖縄 戸篠 祥．2017．日本産箱虫綱の分類と生活史．クラゲ類の生態学的研究（豊川雅哉，西川 淳，三宅裕志編），pp. 2–16. 生物研究社，東京 Hifumi, T., Fukuchi, Y., Otani, N., Kondo, Y., Kitamoto, T., Kobayashi, K., Nakaya, N. and Tomioka, J. 2019. Clinical characteristics of jellysh stings in Japan. Acute Med. Surg., 7: e469. 山口正士．1982．立方クラゲ類とその生活史．海洋と生物，21: 248–254. Bolte, B., Goldsbury, J., Huerlimann, R., Jerry, D. and Kingsford, M. 2021. Validation of eDNA as a viable method of detection for dangerous cubozoan jellyfish. Environ. DNA, 3: 769–779. 岩永節子，大城直雅．2007．宜野湾マリーナにおけるハブクラゲの終日観察．沖縄県衛生環境研究所報，41: 101–102. Minamoto, T., Fukuda, M., Katsuhara, K. R., Fujiwara, A., Hidaka, S., Yamamoto, S., Takahashi, K. and Masuda, R. 2017. Environmental DNA reflects spatial and temporal jellyfish distribution. PLoS One, 12: e0173073. Sathirapongsasuti, N., Khonchom, K., Poonsawat, T., Pransilpa, M., Ongsara, S., Detsri, U., Bungbai, S., Lawanangkoon, S., Pattanaporkrattana, W. and Trakulsrichai, S. 2021. Rapid and Accurate Species-Specific PCR for the Identification of Lethal Chironex Box Jellyfish in Thailand. Int. J. Environ. Res. Public Health, 18: 219. Lewis, C. and Bentlage, B. 2009. Clarifying the identity of the Japanese Habu-kurage, Chironex yamaguchii, sp nov (Cnidaria: Cubozoa: Chirodropida). Zootaxa, 2030: 59–65. 山中裕樹，廣原嵩也．2019．環境DNA試料の採取から分析に至るまで 採水，保存・運搬，ろ過の現状．化学と生物，57: 380–387. 上里 博．2012．海洋危険生物による皮膚障害（I）．西日本皮膚科，74: 519–540. R Core Team. 2022. R: A language and environment for statistical computing. R Foundation for Statistical Computing, Vienna, Austria. [accessed October 15, 2022] Available from: URL https://www.R-project.org Untergasser, A., Nijveen, H., Rao, X., Bisseling, T., Geurts, R. and Leunissen, J. A. 2007. Primer3Plus, an enhanced web interface to Primer3. Nucleic Acids Res., 35(suppl_2): W71–W74. Brinkman, D. L. and Burnell, J. N. 2009. Biochemical and molecular characterisation of cubozoan protein toxins. Toxicon, 54: 1162–1173. 岩永節子，大城直雅，前之園唯史，仲宗根民男．2001．沖縄島におけるハブクラゲの季節消長．平成11–12年度 海洋性有害生物実態調査報告書，pp. 31–33. 沖縄県衛生環境研究所，沖縄 Minamoto, T., Miya, M., Sado, T., Seino, S., Doi, H., Kondoh, M., Nakamura, K., Takahara, T., Yamamoto, S., Yamanaka, H., Araki, H., Iwasaki, W., Kasai, A., Masuda, R. and Uchii, K. 2021. An illustrated manual for environmental DNA research: water sampling guidelines and experimental protocols. Environ. DNA, 3: 8–13. Murakami, H., Yoon, S., Kasai, A., Minamoto, T., Yamamoto, S., Sakata, K. M., Horiuchi, T., Sawada, H., Kondoh, M., Yamashita, Y. and Masuda, R. 2019. Dispersion and degradation of environmental DNA from caged fish in a marine environment. Fish. Sci., 85: 327–337. Kumar, S., Stecher, G., Li, M., Knyaz, C. and Tamura, K. 2018. MEGA X: molecular evolutionary genetics analysis across computing platforms. Mol. Biol. Evol., 35: 1547–1549. Folmer, O., Black, M., Hoeh, W., Lutz, R. and Vrigenhoek, R. 1994. DNA primers for amplification of mitochondrial cytochrome c oxidase subunit I from diverse metazoan invertebrates. Mol. Mar. Biol. Biotechnol., 3: 294–299. 沖縄県衛生環境研究所．2012．沖縄県における海洋危険生物による刺咬症被害—1998～2010—．平成22–23年度ハブクラゲ等危害防止対策事業報告書，pp. 24–38. Takasu, H., Inomata, H., Uchino, K., Tahara, S., Mori, K., Hirano, Y., Kana Harada, K., Yamaguchi, M., Nozoe, Y. and Akiyama, H. 2019. Spatio-temporal distribution of environmental DNA derived from Japanese sea nettle jellyfish Chrysaora pacifica in Omura Bay, Kyushu, Japan. Plankton Benthos Res., 14: 320–323. 坂田雅之，徐 寿明，源 利文．2021．環境DNA分析の概要．環境DNA生態系の真の姿を読み解く（土井秀幸，近藤倫生編），pp. 1–35. 共立出版，東京 Ames, C. L., Ohdera, A. H., Colston, S. M., Collins, A. G., Fitt, W. K., Morandini, A. C., Erickson, J. S. and Vora, G. J. 2021. Fieldable environmental DNA sequencing to assess jellyfish biodiversity in nearshore waters of the Florida Keys, United States. Front. Mar. Sci., 8: 640527. 岩永節子，城間 侔，勝連盛輝，金本昭彦，大場淳子，野崎真敏．2006．ハブクラゲ等海洋有毒生物の生態と防除に関する調査研究．平成17年度 亜熱帯地域の有害・有毒生物に関する調査研究報告書，pp. 55–78. 財団法人 亜熱帯総合研究所，沖縄 岩永節子，城間 侔，金本昭彦，大場淳子，勝連盛輝，岡田 敏．2005．ハブクラゲ等海洋有毒生物の生態と防除に関する調査研究．平成16年度 亜熱帯地域の有害・有毒生物に関する調査研究報告書，pp. 65–92. 財団法人 亜熱帯総合研究所，沖縄 Carrette, T. J. and Seymour, J. J. 2013. Long-term analysis of Irukandji stings in Far North Queensland. Diving Hyperb. Med., 43: 9–15. Gaynor, J. J., Bologna, P. A., Restaino, D. J. and Barry, C. L. 2017. qPCR detection of early life history stage Chrysaora quinquecirrha (sea nettles) in Barnegat Bay, New Jersey. J. Coast. Res., 78: 184–192. Oka, S., Doi, H., Miyamoto, K., Hanahara, N., Sado, T. and Miya, M. 2020. Environmental DNA metabarcoding for biodiversity monitoring of a highly diverse tropical fish community in a coral reef lagoon: Estimation of species richness and detection of habitat segregation. Environ. DNA, 3: 55–69. タカラバイオ株式会社．2009.リアルタイムPCR実験ガイド リアルタイムPCR実践編—プライマー設計ガイドライン—. [accessed October 9, 2022] Available from: https://www.takara-bio.co.jp/research/prt/pdfs/prt3-1.pdf 山中裕樹．2021．種特異的環境DNA手法．環境DNA生態系の真の姿を読み解く（土井秀幸，近藤倫生 編），pp. 36–55. 共立出版，東京 22 23 24 25 26 27 28 29 30 31 10 32 11 33 12 13 14 15 16 17 18 19 1 2 3 4 5 6 7 8 9 20 21 |
| References_xml | – reference: Lewis, C. and Bentlage, B. 2009. Clarifying the identity of the Japanese Habu-kurage, Chironex yamaguchii, sp nov (Cnidaria: Cubozoa: Chirodropida). Zootaxa, 2030: 59–65. – reference: Bolte, B., Goldsbury, J., Huerlimann, R., Jerry, D. and Kingsford, M. 2021. Validation of eDNA as a viable method of detection for dangerous cubozoan jellyfish. Environ. DNA, 3: 769–779. – reference: Minamoto, T., Fukuda, M., Katsuhara, K. R., Fujiwara, A., Hidaka, S., Yamamoto, S., Takahashi, K. and Masuda, R. 2017. Environmental DNA reflects spatial and temporal jellyfish distribution. PLoS One, 12: e0173073. – reference: タカラバイオ株式会社．2009.リアルタイムPCR実験ガイド リアルタイムPCR実践編—プライマー設計ガイドライン—. [accessed October 9, 2022] Available from: https://www.takara-bio.co.jp/research/prt/pdfs/prt3-1.pdf – reference: Murakami, H., Yoon, S., Kasai, A., Minamoto, T., Yamamoto, S., Sakata, K. M., Horiuchi, T., Sawada, H., Kondoh, M., Yamashita, Y. and Masuda, R. 2019. Dispersion and degradation of environmental DNA from caged fish in a marine environment. Fish. Sci., 85: 327–337. – reference: 山中裕樹，廣原嵩也．2019．環境DNA試料の採取から分析に至るまで 採水，保存・運搬，ろ過の現状．化学と生物，57: 380–387. – reference: 岩永節子，大城直雅，前之園唯史，仲宗根民男．2001．沖縄島におけるハブクラゲの季節消長．平成11–12年度 海洋性有害生物実態調査報告書，pp. 31–33. 沖縄県衛生環境研究所，沖縄． – reference: Minamoto, T., Miya, M., Sado, T., Seino, S., Doi, H., Kondoh, M., Nakamura, K., Takahara, T., Yamamoto, S., Yamanaka, H., Araki, H., Iwasaki, W., Kasai, A., Masuda, R. and Uchii, K. 2021. An illustrated manual for environmental DNA research: water sampling guidelines and experimental protocols. Environ. DNA, 3: 8–13. – reference: Folmer, O., Black, M., Hoeh, W., Lutz, R. and Vrigenhoek, R. 1994. DNA primers for amplification of mitochondrial cytochrome c oxidase subunit I from diverse metazoan invertebrates. Mol. Mar. Biol. Biotechnol., 3: 294–299. – reference: 沖縄環境保全研究所．2008．平成19年度ハブクラゲ分布調査及び採集・輸送業務報告書，154 pp. – reference: Carrette, T. J. and Seymour, J. J. 2013. Long-term analysis of Irukandji stings in Far North Queensland. Diving Hyperb. Med., 43: 9–15. – reference: 山中裕樹．2021．種特異的環境DNA手法．環境DNA生態系の真の姿を読み解く（土井秀幸，近藤倫生 編），pp. 36–55. 共立出版，東京． – reference: Sathirapongsasuti, N., Khonchom, K., Poonsawat, T., Pransilpa, M., Ongsara, S., Detsri, U., Bungbai, S., Lawanangkoon, S., Pattanaporkrattana, W. and Trakulsrichai, S. 2021. Rapid and Accurate Species-Specific PCR for the Identification of Lethal Chironex Box Jellyfish in Thailand. Int. J. Environ. Res. Public Health, 18: 219. – reference: 戸篠 祥．2017．日本産箱虫綱の分類と生活史．クラゲ類の生態学的研究（豊川雅哉，西川 淳，三宅裕志編），pp. 2–16. 生物研究社，東京． – reference: Gaynor, J. J., Bologna, P. A., Restaino, D. J. and Barry, C. L. 2017. qPCR detection of early life history stage Chrysaora quinquecirrha (sea nettles) in Barnegat Bay, New Jersey. J. Coast. Res., 78: 184–192. – reference: Takasu, H., Inomata, H., Uchino, K., Tahara, S., Mori, K., Hirano, Y., Kana Harada, K., Yamaguchi, M., Nozoe, Y. and Akiyama, H. 2019. Spatio-temporal distribution of environmental DNA derived from Japanese sea nettle jellyfish Chrysaora pacifica in Omura Bay, Kyushu, Japan. Plankton Benthos Res., 14: 320–323. – reference: 岩永節子，城間 侔，金本昭彦，大場淳子，勝連盛輝，岡田 敏．2005．ハブクラゲ等海洋有毒生物の生態と防除に関する調査研究．平成16年度 亜熱帯地域の有害・有毒生物に関する調査研究報告書，pp. 65–92. 財団法人 亜熱帯総合研究所，沖縄． – reference: Untergasser, A., Nijveen, H., Rao, X., Bisseling, T., Geurts, R. and Leunissen, J. A. 2007. Primer3Plus, an enhanced web interface to Primer3. Nucleic Acids Res., 35(suppl_2): W71–W74. – reference: 岩永節子，城間 侔，勝連盛輝，金本昭彦，大場淳子，野崎真敏．2006．ハブクラゲ等海洋有毒生物の生態と防除に関する調査研究．平成17年度 亜熱帯地域の有害・有毒生物に関する調査研究報告書，pp. 55–78. 財団法人 亜熱帯総合研究所，沖縄． – reference: 坂田雅之，徐 寿明，源 利文．2021．環境DNA分析の概要．環境DNA生態系の真の姿を読み解く（土井秀幸，近藤倫生編），pp. 1–35. 共立出版，東京． – reference: Jo, T., Murakami, H., Yamamoto, S., Masuda, R. and Minamoto, T. 2019. Effect of water temperature and fish biomass on environmental DNA shedding, degradation, and size distribution. Ecol. Evol., 9: 1135–1146. – reference: Oka, S., Doi, H., Miyamoto, K., Hanahara, N., Sado, T. and Miya, M. 2020. Environmental DNA metabarcoding for biodiversity monitoring of a highly diverse tropical fish community in a coral reef lagoon: Estimation of species richness and detection of habitat segregation. Environ. DNA, 3: 55–69. – reference: Kumar, S., Stecher, G., Li, M., Knyaz, C. and Tamura, K. 2018. MEGA X: molecular evolutionary genetics analysis across computing platforms. Mol. Biol. Evol., 35: 1547–1549. – reference: 沖縄県衛生環境研究所．2012．沖縄県における海洋危険生物による刺咬症被害—1998～2010—．平成22–23年度ハブクラゲ等危害防止対策事業報告書，pp. 24–38. – reference: Kawamura, M., Ueno, S., Iwanaga, S., Oshiro, N. and Kubota, S. 2003. The relationship between fine rings in the statolith and growth of the cubomedusa Chiropsalmus quadrigatus (Cnidaria: Cubozoa) from Okinawa Island, Japan. Plankton Biol. Ecol., 50: 37–42. – reference: 岩永節子，金本昭彦．2004．ハブクラゲ等海洋有毒生物の生態と防除に関する調査研究．平成15年度 亜熱帯地域の有害・有毒生物に関する調査研究報告書，pp.57–83. 財団法人 亜熱帯総合研究所，沖縄． – reference: R Core Team. 2022. R: A language and environment for statistical computing. R Foundation for Statistical Computing, Vienna, Austria. [accessed October 15, 2022] Available from: URL https://www.R-project.org/ – reference: 山口正士．1982．立方クラゲ類とその生活史．海洋と生物，21: 248–254. – reference: 上里 博．2012．海洋危険生物による皮膚障害（I）．西日本皮膚科，74: 519–540. – reference: 岩永節子，大城直雅．2007．宜野湾マリーナにおけるハブクラゲの終日観察．沖縄県衛生環境研究所報，41: 101–102. – reference: Brinkman, D. L. and Burnell, J. N. 2009. Biochemical and molecular characterisation of cubozoan protein toxins. Toxicon, 54: 1162–1173. – reference: Hifumi, T., Fukuchi, Y., Otani, N., Kondo, Y., Kitamoto, T., Kobayashi, K., Nakaya, N. and Tomioka, J. 2019. Clinical characteristics of jellysh stings in Japan. Acute Med. Surg., 7: e469. – reference: Ames, C. L., Ohdera, A. H., Colston, S. M., Collins, A. G., Fitt, W. K., Morandini, A. C., Erickson, J. S. and Vora, G. J. 2021. Fieldable environmental DNA sequencing to assess jellyfish biodiversity in nearshore waters of the Florida Keys, United States. Front. Mar. Sci., 8: 640527. – ident: 15 doi: 10.1093/molbev/msy096 – ident: 4 – ident: 12 – ident: 26 doi: 10.3800/pbr.14.320 – ident: 10 – ident: 30 doi: 10.1093/nar/gkm306 – ident: 1 doi: 10.3389/fmars.2021.640527 – ident: 14 – ident: 31 – ident: 28 – ident: 25 doi: 10.3390/ijerph18010219 – ident: 7 doi: 10.1002/ams2.469 – ident: 18 doi: 10.1002/edn3.121 – ident: 13 doi: 10.1002/ece3.4802 – ident: 20 doi: 10.1002/edn3.132 – ident: 3 doi: 10.1016/j.toxicon.2009.02.006 – ident: 24 – ident: 9 – ident: 19 doi: 10.1007/s12562-018-1282-6 – ident: 22 – ident: 17 doi: 10.1371/journal.pone.0173073 – ident: 5 – ident: 11 – ident: 33 doi: 10.1271/kagakutoseibutsu.57.380 – ident: 29 doi: 10.2336/nishinihonhifu.74.519 – ident: 32 – ident: 8 – ident: 16 doi: 10.11646/zootaxa.2030.1.5 – ident: 21 – ident: 2 doi: 10.1002/edn3.181 – ident: 6 doi: 10.2112/SI78-014.1 – ident: 27 – ident: 23 |
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| Title | Real-time PCR assay for detection of Environmental DNA from Chironex yamaguchii |
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