Evaluation of three methods for detecting bacterial contamination in intravenous solutions

• Published in: American journal of hospital pharmacy Vol. 39; no. 8; p. 1299
• Main Authors: Hoffman, K H, Smith, F M, Godwin, H N, Hogan, L C, Furtado, D
• Format: Journal Article
• Language: English
• Published: United States 01.08.1982
• Subjects: Bacteriological Techniques; Drug Contamination; Evaluation Studies as Topic; Injections, Intravenous - standards; Solutions - standards; Staphylococcus - isolation & purification; Sterilization

Membrane filtration, small-aliquot inoculation, and double-strength broth methods of sterility testing were evaluated for detection of small numbers of bacteria in 5% dextrose injection (D5W). Each of 240 bags of D5W 50 ml were inoculated with approximately 10 2 Staphylococcus epidermidis and subjec...