The crystal structure of coenzyme B 12 ‐dependent glycerol dehydratase in complex with cobalamin and propane‐1,2‐diol

• Published in: European journal of biochemistry Vol. 269; no. 18; pp. 4484 - 4494
• Main Authors: Yamanishi, Mamoru, Yunoki, Michio, Tobimatsu, Takamasa, Sato, Hideaki, Matsui, Junko, Dokiya, Ayako, Iuchi, Yasuhiro, Oe, Kazunori, Suto, Kyoko, Shibata, Naoki, Morimoto, Yukio, Yasuoka, Noritake, Toraya, Tetsuo
• Format: Journal Article
• Language: English
• Published: 01.09.2002

Recombinant glycerol dehydratase of Klebsiella pneumoniae was purified to homogeneity. The subunit composition of the enzyme was most probably α 2 β 2 γ 2 . When ( R )‐ and ( S )‐propane‐1,2‐diols were used independently as substrates, the rate with the ( R )‐enantiomer was 2.5 times faster than tha...