Detection of Bacteroides forsythus in Human Subgingival Flora by Using Bacterial Culture and a DNA Probe, and Its Relationship to Periodontal Diseases
The purpose of this investigation was to evaluate the role of Bacteroides forsythus (B. forsythus) in the pathogenesis of periodontal disease. Bacterial cul-ture and DNA probe analysis were performed to detect B. forsythus in subgingival plaque samples obtained from healthy control subjects and pati...
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| Published in | Nihon Shishubyo Gakkai Kaishi (Journal of the Japanese Society of Periodontology) Vol. 39; no. 1; pp. 1 - 11 |
|---|---|
| Main Author | |
| Format | Journal Article |
| Language | Japanese |
| Published |
JAPANESE SOCIETY OF PERIODONTOLOGY
1997
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| Subjects | |
| Online Access | Get full text |
| ISSN | 0385-0110 1880-408X |
| DOI | 10.2329/perio.39.1 |
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| Abstract | The purpose of this investigation was to evaluate the role of Bacteroides forsythus (B. forsythus) in the pathogenesis of periodontal disease. Bacterial cul-ture and DNA probe analysis were performed to detect B. forsythus in subgingival plaque samples obtained from healthy control subjects and patients with adult, and/or early-onset periodontitis. Bac-terial culture and subculture of type strains and clinical isolates of B. forsythus became easier when N-acetylmuramic acid was applied to trypticase soy blood agar at a concentration of 1mg/l. Affirm DP ® (Becton Dickinson, USA) was em-ployed for DNA probe analysis, and this system was found to be capable of detecting more than105 bacterial cells. After the standard methods of bacterial culture and the DNA probe method were established, the prevalence of B. forsythus in subgin-gival plaque from various types of periodontal disease was investigated. The results showed that B. forsythus was seldom detected in the gingival sulcus in the healthy control group, but was detect-ed at a much higher ratio in the pockets ofperi-odontitis group by both methods. Furthermore, the prevalence and total cultivable number of B. forsy-thus weresignificantly correlated with probing pocket depth, bleeding on probing, and degree of bone loss, suggesting that B. forsythus is plays a large role in the pathogenesis ofperiodontitis |
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| AbstractList | The purpose of this investigation was to evaluate the role of Bacteroides forsythus (B. forsythus) in the pathogenesis of periodontal disease. Bacterial cul-ture and DNA probe analysis were performed to detect B. forsythus in subgingival plaque samples obtained from healthy control subjects and patients with adult, and/or early-onset periodontitis. Bac-terial culture and subculture of type strains and clinical isolates of B. forsythus became easier when N-acetylmuramic acid was applied to trypticase soy blood agar at a concentration of 1mg/l. Affirm DP ® (Becton Dickinson, USA) was em-ployed for DNA probe analysis, and this system was found to be capable of detecting more than105 bacterial cells. After the standard methods of bacterial culture and the DNA probe method were established, the prevalence of B. forsythus in subgin-gival plaque from various types of periodontal disease was investigated. The results showed that B. forsythus was seldom detected in the gingival sulcus in the healthy control group, but was detect-ed at a much higher ratio in the pockets ofperi-odontitis group by both methods. Furthermore, the prevalence and total cultivable number of B. forsy-thus weresignificantly correlated with probing pocket depth, bleeding on probing, and degree of bone loss, suggesting that B. forsythus is plays a large role in the pathogenesis ofperiodontitis |
| Author | Yano, Kazuko |
| Author_xml | – sequence: 1 fullname: Yano, Kazuko organization: Department of Periodontology, Faculty of Dentistry |
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| References | Kerosuo, E.: Ultrastructure of the cell envelope of Bacteroides forsythus strain ATCC 43037T. Oral. Microbiol. Immunol., 3: 134-137, 1988 Wyss, C.: Dependence of proliferation of Bacteroides forsythus on exogenous N -acetylmuramic acid. Infect. Immun., 57: 1757-1759, 1989. 矢野和子, 高松伸博, 何濤, 梅田誠, 石川烈: 非放射性DNAプローブ法 (AffirmDP (R)) の歯周病原性細菌の検出能について. 口病誌., 63: 482-488, 1996. Savit, E. D. and Socransky, S. S.: Distribution of certain subgingival microbial species in selected periodontal conditions. J. Periodont. Res., 19: 111-122, 1982. Ali, R. W., Bakken, V., Nilsen, R. and Skaug, N.: Comparative detection frequency of 6 putative periodontal pathogens in Sudanese and Norwegian adult periodontitis patients. J. Periodontol., 65: 1046-1052, 1994. Zambon, J. J.: Actinobacillus actinomycetemcomitans in human periodontal disease. J. Clin. Periodontol., 12: 1-20, 1985. Tanner, A. C. R., Haffer, C., Bratthall, G. T., Visconti, R. A. and Socransky, S. S.: A study of bacteria associated with advancing periodontitis in man. J. Clin. Periodontol., 6: 278-307, 1979. Moncla, B. J., Motley, S. T., Braham, P., Ewing, L., Adams, T. H. and Vermeulen, N. M. J.: Use of synthetic oligonucleotide DNA probes for identification and direct detection of Bacteroides forsythus in plaque samples. J. Clin. Microbiol., 29: 2158-2162, 1991. Ishihara, K., Naito, Y., Kato, T., Takazoe, I., Okuda, K., Eguchi, T., Nakashima, K., Matsuda, N., Yamasaki, K., Hasegawa, K., Suido, H. and Sugihara, K.: A sensitive enzymatic method (SK -013) for detection and quantification of specific periodontopathogens. J. Periodont. Res., 27: 81-85, 1992. Grossi, S. G., Genco, R. J., Machtei, E. E., Ho, A. W., Koch, G., Dunford, R., Zambon, J. J. and Hausmann, E.: Assessment of risk for periodontal disease. II. Risk indicators for alveolar bone loss. J. Periodontol., 66: 23-29, 1995. Kamma, J. J., Nakou, M. and Manti, F. A.: Microbiota of rapidly progressive periodontitis lesions in association with clinical parameters. J. Periodontal. . 65: 1073-1078. 1994. Lotufo, R. F. M., Flynn, J., Chen, C. and Slots, J.: Molecular detection of Bacteroides forsythus in human periodontitis. Oral. Microbiol. Immunol., 9: 154-160, 1994. Lai, C-H., Listgarten, M. A., Shirakawa, M. and Slots, J.: Bacteroides forsythus in adult gingivitis and periodontitis. Oral. Microbiol. Immunol., 2: 152-157, 1987. Slots, J .: Selective medium for isolation of Actinobacillus actinornycetencomitans. J. Clin. Microbiol., 15: 606-609, 1982. Gmtir, R. and Guggenheim, B.: Monoclonal antibodies for the detection of 'peiodontopathic' bacteria. Arch. Oral. Biol., 35: 145-151, 1990. Umeda, M., Ishikawa, I., Benno, Y. and Mitsuoka, T.: Improved detection of oral spirochtes with an anaerobic culture method. Oral. Microbiol. Immunol., 5: 90-94, 1990. 穂坂康朗: 初期治療における血清及び歯齪溝滲出液中の歯周病原性細菌に対するIgG抗体価の変動について. 日歯周誌, 34: 1-19, 1992. Tanner, A. and Bouldin, H.: The microbiota of early periodontitis lesions in adults. J. Clin. Periodontol., 16: 467-471, 1989. Rams, T. E., Feik, D. and Slots, J.: Campylobacter rectus in human periodontitis. Oral. Microbiol. Immunol., 8: 230-235, 1993. Dzink, J. L., Tanner, A. C. R., Haffajee, A. D. and Socransky, S. S.: Gram negative species associated with active destructive periodontal lesions. J. Clin. Periodontol., 12: 648-659, 1985. Werner-Felmayer, G., Guggenheim, B. and Gmiir, R.: Production and characterization of monoclonal antibodies against Bacteroides forsythus and Wolinella recta. J. Dent. Res., 67: 548-553, 1988. Moncla, B. J., Braham, P., Rabe, L. K. and Hillier, S. L.: Rapid presumptive identification of blackpigmented gram-negative anaerobic bacteria by using 4-methylumbelliferone derivatives. J. Clin. Microbiol., 29: 1955-1958. 1991. Tanner, A., Bouldin, H. D. and Maiden, M. F.: Newly delineated periodontal pathogens with special reference to selenomonas species. Infection, 17: 182-187, 1989. Seida, K., Kato, T., Yamada, S., Ishihara, K., Naito, Y. and Okuda, K.: A sensitive enzymatic method (SK-013) for detection of Treponema denticola, Porphyromonas gingivalis and Bacteroides forsythus in subgingival plaque samples. J. Periodont. Res., 27: 86-91, 1992. Loesche, W. J., Bretz, W. A., Lopatin, D., Stoll, J., Rau, C. F., Hillenburg, K. L., Killoy, W. J., Drisko, C. L., Williams, R., Weber, H. P., Clark, W., Magnusson, I., Walker, C. and Hujoel, P. P.: Multi-center clinical evaluation of a chairside method for detecting certain periodontopathic bacteria in periodontal disease. J. Periodontol., 61: 189-196, 1990. Gmtir, R., Strub, J. R. and Guggenheim, B.: Prevalence of Bacteroides forsythus and Bacteroides gingivalis in subgingival plaque of prosthodontically treated patients on short recall. J. Periodont. Res., 24: 113-20, 1989. Zambon, J. J.: Microbiology of periodontal disease. in: Contemporary Periodontics (Genco, R. J., Coldman, H. M. and Cohen, D. W. ed.), Mosby, St. Louis, 147-160, 1990. Umeda, M., Tominaga, Y., He, T., Yano, K., Watanabe, H. and Ishikawa, I.: Microbial flora in the acute phase of periodontitis and the effect of local administration of minocycline. J. Periodontol., 67: 422-427, 1996. Gersdorf, H., Meissner, A., Peltz, K., Krekeler, G. and GObel, U. B.: Idetification of Bacteroides forsythus in subgingival plaque from patients with advanced periodontitis. J. Clin. Microbiol., 31: 941-946, 1993. Grossi, S. G., Zambon, J. J., Ho, A. W., Koch, G., Dunford, R. G., Machtei, E. E., Norderyd, O. M. and Genco, R. J.: Assessment of risk for periodontal disease. I. Risk indicators for attachment loss. J. Periodontol., 65: 260-267, 1994. Braham, P. H. and Moncla, B. J.: Rapid presumptive identification and further characterization of Bacteroides forsythus. J. Clin. Microbiol., 30: 649-654. 1992. Tanner, A. C. R., Listgarten, M. A., Ebersole, J. L. and Strzempko, M. N.: Bacteroides forsythus sp. nov., a slow-growing, fusiform Bacteroides sp. from the human oral cavity. Int. J. Syst. Bacteriol., 36: 213-221, 1986. Ali, R. W., Velcescu, C., Jivanscu, M-C., Lofthus, B. and Skaug, N.: Prevalence of 6 putative periodontal pathogens in subgingival plaque samples from Romanian adult periodontitis patients. J. Clin. Periodontol., 23: 133-139, 1996. Listgarten, M. A., Wong, M. -Y. and Lai, C. -H.: Detection of Actinobacillus actinornyceterncomitans, Porphyrornonas gingivalis, and Bacteroides forsythus in an A. actinornyceterncornitans-positive patient population. J. Periodontol., 66: 158-164, 1995. Listgarten, M. A., Lai, C. -H. and Young, V.: Microbial composition and pattern of antibiotic resistance in subgingival microbial samples from patients with refractory periodontitis. J. Periodontol., 64: 155-161, 1993. |
| References_xml | – reference: Gersdorf, H., Meissner, A., Peltz, K., Krekeler, G. and GObel, U. B.: Idetification of Bacteroides forsythus in subgingival plaque from patients with advanced periodontitis. J. Clin. Microbiol., 31: 941-946, 1993. – reference: Gmtir, R. and Guggenheim, B.: Monoclonal antibodies for the detection of 'peiodontopathic' bacteria. Arch. Oral. Biol., 35: 145-151, 1990. – reference: Ali, R. W., Velcescu, C., Jivanscu, M-C., Lofthus, B. and Skaug, N.: Prevalence of 6 putative periodontal pathogens in subgingival plaque samples from Romanian adult periodontitis patients. J. Clin. Periodontol., 23: 133-139, 1996. – reference: Ali, R. W., Bakken, V., Nilsen, R. and Skaug, N.: Comparative detection frequency of 6 putative periodontal pathogens in Sudanese and Norwegian adult periodontitis patients. J. Periodontol., 65: 1046-1052, 1994. – reference: Wyss, C.: Dependence of proliferation of Bacteroides forsythus on exogenous N -acetylmuramic acid. Infect. Immun., 57: 1757-1759, 1989. – reference: Kamma, J. J., Nakou, M. and Manti, F. A.: Microbiota of rapidly progressive periodontitis lesions in association with clinical parameters. J. Periodontal. . 65: 1073-1078. 1994. – reference: Slots, J .: Selective medium for isolation of Actinobacillus actinornycetencomitans. J. Clin. Microbiol., 15: 606-609, 1982. – reference: Rams, T. E., Feik, D. and Slots, J.: Campylobacter rectus in human periodontitis. Oral. Microbiol. Immunol., 8: 230-235, 1993. – reference: Werner-Felmayer, G., Guggenheim, B. and Gmiir, R.: Production and characterization of monoclonal antibodies against Bacteroides forsythus and Wolinella recta. J. Dent. Res., 67: 548-553, 1988. – reference: Tanner, A. and Bouldin, H.: The microbiota of early periodontitis lesions in adults. J. Clin. Periodontol., 16: 467-471, 1989. – reference: Tanner, A. C. R., Listgarten, M. A., Ebersole, J. L. and Strzempko, M. N.: Bacteroides forsythus sp. nov., a slow-growing, fusiform Bacteroides sp. from the human oral cavity. Int. J. Syst. Bacteriol., 36: 213-221, 1986. – reference: Zambon, J. J.: Actinobacillus actinomycetemcomitans in human periodontal disease. J. Clin. Periodontol., 12: 1-20, 1985. – reference: Lotufo, R. F. M., Flynn, J., Chen, C. and Slots, J.: Molecular detection of Bacteroides forsythus in human periodontitis. Oral. Microbiol. Immunol., 9: 154-160, 1994. – reference: Kerosuo, E.: Ultrastructure of the cell envelope of Bacteroides forsythus strain ATCC 43037T. Oral. Microbiol. Immunol., 3: 134-137, 1988 – reference: Ishihara, K., Naito, Y., Kato, T., Takazoe, I., Okuda, K., Eguchi, T., Nakashima, K., Matsuda, N., Yamasaki, K., Hasegawa, K., Suido, H. and Sugihara, K.: A sensitive enzymatic method (SK -013) for detection and quantification of specific periodontopathogens. J. Periodont. Res., 27: 81-85, 1992. – reference: Loesche, W. J., Bretz, W. A., Lopatin, D., Stoll, J., Rau, C. F., Hillenburg, K. L., Killoy, W. J., Drisko, C. L., Williams, R., Weber, H. P., Clark, W., Magnusson, I., Walker, C. and Hujoel, P. P.: Multi-center clinical evaluation of a chairside method for detecting certain periodontopathic bacteria in periodontal disease. J. Periodontol., 61: 189-196, 1990. – reference: Dzink, J. L., Tanner, A. C. R., Haffajee, A. D. and Socransky, S. S.: Gram negative species associated with active destructive periodontal lesions. J. Clin. Periodontol., 12: 648-659, 1985. – reference: Moncla, B. J., Braham, P., Rabe, L. K. and Hillier, S. L.: Rapid presumptive identification of blackpigmented gram-negative anaerobic bacteria by using 4-methylumbelliferone derivatives. J. Clin. Microbiol., 29: 1955-1958. 1991. – reference: Listgarten, M. A., Wong, M. -Y. and Lai, C. -H.: Detection of Actinobacillus actinornyceterncomitans, Porphyrornonas gingivalis, and Bacteroides forsythus in an A. actinornyceterncornitans-positive patient population. J. Periodontol., 66: 158-164, 1995. – reference: Umeda, M., Ishikawa, I., Benno, Y. and Mitsuoka, T.: Improved detection of oral spirochtes with an anaerobic culture method. Oral. Microbiol. Immunol., 5: 90-94, 1990. – reference: Savit, E. D. and Socransky, S. S.: Distribution of certain subgingival microbial species in selected periodontal conditions. J. Periodont. Res., 19: 111-122, 1982. – reference: Moncla, B. J., Motley, S. T., Braham, P., Ewing, L., Adams, T. H. and Vermeulen, N. M. J.: Use of synthetic oligonucleotide DNA probes for identification and direct detection of Bacteroides forsythus in plaque samples. J. Clin. Microbiol., 29: 2158-2162, 1991. – reference: Zambon, J. J.: Microbiology of periodontal disease. in: Contemporary Periodontics (Genco, R. J., Coldman, H. M. and Cohen, D. W. ed.), Mosby, St. Louis, 147-160, 1990. – reference: Listgarten, M. A., Lai, C. -H. and Young, V.: Microbial composition and pattern of antibiotic resistance in subgingival microbial samples from patients with refractory periodontitis. J. Periodontol., 64: 155-161, 1993. – reference: Tanner, A. C. R., Haffer, C., Bratthall, G. T., Visconti, R. A. and Socransky, S. S.: A study of bacteria associated with advancing periodontitis in man. J. Clin. Periodontol., 6: 278-307, 1979. – reference: Tanner, A., Bouldin, H. D. and Maiden, M. F.: Newly delineated periodontal pathogens with special reference to selenomonas species. Infection, 17: 182-187, 1989. – reference: Grossi, S. G., Zambon, J. J., Ho, A. W., Koch, G., Dunford, R. G., Machtei, E. E., Norderyd, O. M. and Genco, R. J.: Assessment of risk for periodontal disease. I. Risk indicators for attachment loss. J. Periodontol., 65: 260-267, 1994. – reference: Grossi, S. G., Genco, R. J., Machtei, E. E., Ho, A. W., Koch, G., Dunford, R., Zambon, J. J. and Hausmann, E.: Assessment of risk for periodontal disease. II. Risk indicators for alveolar bone loss. J. Periodontol., 66: 23-29, 1995. – reference: Braham, P. H. and Moncla, B. J.: Rapid presumptive identification and further characterization of Bacteroides forsythus. J. Clin. Microbiol., 30: 649-654. 1992. – reference: Umeda, M., Tominaga, Y., He, T., Yano, K., Watanabe, H. and Ishikawa, I.: Microbial flora in the acute phase of periodontitis and the effect of local administration of minocycline. J. Periodontol., 67: 422-427, 1996. – reference: Gmtir, R., Strub, J. R. and Guggenheim, B.: Prevalence of Bacteroides forsythus and Bacteroides gingivalis in subgingival plaque of prosthodontically treated patients on short recall. J. Periodont. Res., 24: 113-20, 1989. – reference: Lai, C-H., Listgarten, M. A., Shirakawa, M. and Slots, J.: Bacteroides forsythus in adult gingivitis and periodontitis. Oral. Microbiol. Immunol., 2: 152-157, 1987. – reference: 穂坂康朗: 初期治療における血清及び歯齪溝滲出液中の歯周病原性細菌に対するIgG抗体価の変動について. 日歯周誌, 34: 1-19, 1992. – reference: 矢野和子, 高松伸博, 何濤, 梅田誠, 石川烈: 非放射性DNAプローブ法 (AffirmDP (R)) の歯周病原性細菌の検出能について. 口病誌., 63: 482-488, 1996. – reference: Seida, K., Kato, T., Yamada, S., Ishihara, K., Naito, Y. and Okuda, K.: A sensitive enzymatic method (SK-013) for detection of Treponema denticola, Porphyromonas gingivalis and Bacteroides forsythus in subgingival plaque samples. J. Periodont. Res., 27: 86-91, 1992. |
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| Title | Detection of Bacteroides forsythus in Human Subgingival Flora by Using Bacterial Culture and a DNA Probe, and Its Relationship to Periodontal Diseases |
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