Induction of Male-Sterility by Controlling of Gibberellin Biosynthesis in Rice (Oryza sativa)

• Published in: Plant breeding and biotechnology pp. 19 - 29
• Main Authors: 배현경, 오성앵, 박순기
• Format: Journal Article
• Language: English
• Published: 한국육종학회 01.03.2018
• Subjects: 농학
• ISSN: 2287-9358; 2287-9366
• DOI: 10.9787/PBB.2018.6.1.19

Gibberellins (GAs) are important hormones at every plant developmental stage including vegetative and reproductive. It is reported in many plant species that many dwarfism and male sterile mutants have defective GA biosynthesis genes. RNAinterference (RNAi) technology can reduce expression of specific genes at the transcription level. Also, promoters can control thespatial and temporal expression of genes. Specific promoters containing the RNAi vector can control specific genes’ spatial andtemporal expression at the transcriptional level. In this study, at first, five anther specific promoters were chosen and verified byexamining their tissue specific expression using promoter::GUS transgenic analysis. RNAi vectors, which contain five anther specificpromoters and one constitutive promoter fused to GA biosynthesis related genes, were constructed for controlling of GA biosynthesisin stem and floral tissue. OsAct1 promoter containing OsGA20ox1 and OsGA3ox2 RNAi T1 plants showed reduced gene expression andshort plant height phenotype compare to wild type plant. OsGA20ox and OsGA3ox RNAi transformants also showed reduced fertilityor sterile phenotype. KCI Citation Count: 0