A high-quality de novo genome assembly from a single parasitoid wasp

• Published in: bioRxiv
• Main Authors: Ye, Xinhai, Yang, Yi, Tian, Zhaoyang, Xu, Le, Yu, Kaili, Xiao, Shan, Yin, Chuanlin, Xiong, Shijiao, Fang, Qi, Hu, Chen, Li, Fei, Ye, Gongyin
• Format: Paper
• Language: English
• Published: Cold Spring Harbor Cold Spring Harbor Laboratory Press 14.07.2020
• Subjects: Deoxyribonucleic acid; DNA; DNA sequencing; Genomes; Heterozygosity; Nucleotide sequence; Population genetics; Rare species; Synteny

Sequencing and assembling a genome with a single individual have several advantages, such as lower heterozygosity and easier sample preparation. However, the amount of genomic DNA of some small sized organisms might not meet the standard DNA input requirement for current sequencing pipelines. Althou...