1 H detection and dynamic nuclear polarization–enhanced NMR of Aβ 1-42 fibrils

• Published in: Proceedings of the National Academy of Sciences - PNAS Vol. 119; no. 1
• Main Authors: Bahri, Salima, Silvers, Robert, Michael, Brian, Jaudzems, Kristaps, Lalli, Daniela, Casano, Gilles, Ouari, Olivier, Lesage, Anne, Pintacuda, Guido, Linse, Sara, Griffin, Robert G.
• Format: Journal Article
• Language: English
• Published: United States National Academy of Sciences 05.01.2022
• Subjects: Amyloid beta-Peptides - chemistry; Analytical chemistry; Biochemistry, Molecular Biology; Biophysics; Carbon-13 Magnetic Resonance Spectroscopy - methods; Chemical Sciences; Life Sciences; or physical chemistry; Peptide Fragments - chemistry; Protein Conformation; Proton Magnetic Resonance Spectroscopy - methods; Structural Biology; Temperature; Theoretical and; magic-angle spinning; dynamic nuclear polarization; 1H detection; amyloid β1-42

Amyloid-β (Aβ) is the subject of intense scrutiny because of its close association with Alzheimer’s disease (AD), which currently afflicts about 50 million people worldwide. The results reported in this manuscript focus on the new possibilities provided by ultrafast magic-angle spinning (MAS) 1 H de...