A nuclease-dead Cas9-derived tool represses target gene expression

• Published in: Plant physiology (Bethesda) Vol. 195; no. 3; pp. 1880 - 1892
• Main Authors: Wang, Bowen, Liu, Xiaolin, Li, Zhenxiang, Zeng, Kang, Guo, Jiangyi, Xin, Tongxu, Zhang, Zhen, Li, Jian-Feng, Yang, Xueyong
• Format: Journal Article
• Language: English
• Published: United States 28.06.2024
• Subjects: dCas9; transcription repression; CRISPR interference; CRISPR/Cas9; molecular breeding

Manipulation of gene expression is central to understanding gene function, engineering cell behavior, and altering biological traits according to production demands. Nuclease-dead Cas9 (dCas9), a variant of active Cas9, offers a versatile platform for the precise control of genome function without D...