Characterization of recombinant plant cinnamate 4‐hydroxylase produced in yeast

• Published in: European journal of biochemistry Vol. 222; no. 3; pp. 843 - 850
• Main Authors: URBAN, Philippe, WERCK‐REICHHART, Danièle, TEUTSCH, Hermann G., DURST, Francis, REGNIER, Sylvie, KAZMAIER, Michael, POMPON, Denis
• Format: Journal Article
• Language: English; Japanese
• Published: Oxford, UK Blackwell Publishing Ltd 01.06.1994

Helianthus tuberosus cinnamate 4‐hydroxylase (CYP73 or CA4H), a member of the P450 superfamily which catalyses the first oxidative step of the phenylpropanoid pathway in higher plants by transforming cinnamate into p‐coumarate, was expressed in the yeast Saccharomyces cerevisiae. The PCR‐amplified C...