One-carbon metabolism supports S-adenosylmethionine and m6A methylation to control the osteogenesis of bone marrow stem cells and bone formation

• Published in: Journal of bone and mineral research Vol. 39; no. 9; pp. 1356 - 1370
• Main Authors: Zhang, Wenjie, Bai, Yujia, Hao, Lili, Zhao, Yiqing, Zhang, Lujin, Ding, Wenqian, Qi, Yipin, Xu, Qiong
• Format: Journal Article
• Language: English
• Published: England 02.09.2024
• Subjects: Adenosine - analogs & derivatives; Adenosine - metabolism; Adenosine - pharmacology; Animals; Bone Marrow Cells - cytology; Bone Marrow Cells - drug effects; Bone Marrow Cells - metabolism; Carbon - metabolism; Carbon - pharmacology; Glycolysis - drug effects; Hypoxia-Inducible Factor 1, alpha Subunit - metabolism; Male; Mesenchymal Stem Cells - drug effects; Mesenchymal Stem Cells - metabolism; Methotrexate - pharmacology; Methylation - drug effects; Osteogenesis - drug effects; Rats; Rats, Sprague-Dawley; S-Adenosylmethionine - metabolism; S-Adenosylmethionine - pharmacology; M6a; epigenetic reprogramming; osteogenic differentiation; one-carbon metabolism; bone mesenchymal stem cells
• ISSN: 0884-0431; 1523-4681; 1523-4681
• DOI: 10.1093/jbmr/zjae121

The skeleton is a metabolically active organ undergoing continuous remodeling initiated by bone marrow stem cells (BMSCs). Recent research has demonstrated that BMSCs adapt the metabolic pathways to drive the osteogenic differentiation and bone formation, but the mechanism involved remains largely elusive. Here, using a comprehensive targeted metabolome and transcriptome profiling, we revealed that one-carbon metabolism was promoted following osteogenic induction of BMSCs. Methotrexate (MTX), an inhibitor of one-carbon metabolism that blocks S-adenosylmethionine (SAM) generation, led to decreased N6-methyladenosine (m6A) methylation level and inhibited osteogenic capacity. Increasing intracellular SAM generation through betaine addition rescued the suppressed m6A content and osteogenesis in MTX-treated cells. Using S-adenosylhomocysteine (SAH) to inhibit the m6A level, the osteogenic activity of BMSCs was consequently impeded. We also demonstrated that the pro-osteogenic effect of m6A methylation mediated by one-carbon metabolism could be attributed to HIF-1α and glycolysis pathway. This was supported by the findings that dimethyloxalyl glycine rescued the osteogenic potential in MTX-treated and SAH-treated cells by upregulating HIF-1α and key glycolytic enzymes expression. Importantly, betaine supplementation attenuated MTX-induced m6A methylation decrease and bone loss via promoting the abundance of SAM in rat. Collectively, these results revealed that one-carbon metabolite SAM was a potential promoter in BMSC osteogenesis via the augmentation of m6A methylation, and the cross talk between metabolic reprogramming, epigenetic modification, and transcriptional regulation of BMSCs might provide strategies for bone regeneration.