The investigation of the distribution of nerves, blood vessels and immune cells on the fresh human corneal surface using optimized protocols for immunostaining of flat mounted whole cornea

• Published in: Acta ophthalmologica (Oxford, England) Vol. 95; no. S259
• Main Authors: He, Z., Guindolet, D., Forest, F., Cognasse, F., Acquart, S., Perrache, C., Gabison, E., Bergandi, F., Gain, P., Thuret, G.
• Format: Journal Article
• Language: English
• Published: Malden Wiley Subscription Services, Inc 01.09.2017
• Subjects: Actin; Basal cells; Blood vessels; Capillaries; CD4 antigen; CD45 antigen; CD8 antigen; Cell culture; Chromatin; Conjunctiva; Cornea; Cytokeratin; Cytoplasm; E-cadherin; Epithelium; FDA approval; Fibers; Fixatives; Histone H3; Localization; Methanol; Nerves; Neurofilaments; Pax6 protein; Preservation; Proteins; Tubulin; Zonula occludens-1 protein

Purpose To present a reliable protocol for the immunostaining of flat mounted corneas and provide a map of the distribution of nerves, blood vessels and immune cells on fresh human corneal epithelium and conjunctiva. Methods Organ‐cultured human corneas were used for our technique development. 15 fi...