Site‐directed mutagenesis of the ionizable groups in the active site of Zymomonas mobilis pyruvate decarboxylase

• Published in: European journal of biochemistry Vol. 268; no. 12; pp. 3558 - 3565
• Main Authors: Huang, Chang‐Yi, Chang, Alan K., Nixon, Peter F., Duggleby, Ronald G.
• Format: Journal Article
• Language: English; Japanese
• Published: Oxford, UK Blackwell Science Ltd 01.06.2001
• Subjects: catalytic mechanism; pH‐dependence; pyruvate decarboxylase; site‐directed mutagenesis; thiamin diphosphate

Pyruvate decarboxylase (PDC, EC 4.1.1.1) is a thiamin diphosphate‐dependent enzyme about which there is a large body of structural and functional information. The active site contains several absolutely conserved ionizable groups and all of these appear to be important, as judged by the fact that mu...