Different brain structures exhibit the same caffeine levels after the administration of a single dose of caffeine
Caffeine is a highly consumed stimulant of the nervous system. Although caffeine has diverse effects on different brain functions, little is known about the specific pharmacokinetics of this substance in the brain. For instance, most studies that assessed caffeine distribution in the rat brain have...
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Published in | Biosalud : revista ciencias básicas Vol. 15; no. 2; pp. 20 - 27 |
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Main Authors | , , , , , |
Format | Journal Article |
Language | English |
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Universidad de Caldas
01.12.2016
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Abstract | Caffeine is a highly consumed stimulant of the nervous system. Although caffeine has diverse effects on different brain functions, little is known about the specific pharmacokinetics of this substance in the brain. For instance, most studies that assessed caffeine distribution in the rat brain have only measured caffeine levels in the cortex and striatum but not in more specific brain areas.Aims: The purpose of this work was to measure the caffeine concentration in blood and different brain regions (i.e. cortex, striatum, hippocampus, cerebellum and brainstem) at different times after the administration of a single intraperitoneal dose of caffeine.Methods: Adult Wistar rats (250 to 300 gr) were injected with a single intraperitoneal dose of 30 mg/ Kg of caffeine. 20, 40, 60 and 80 minutes after administration, subjects were sacrificed by decapitation and samples of plasma, cerebral cortex, striatum, hippocampus, cerebellum and brainstem were obtained. Caffeine levels in the blood and each brain structure were measured by RP-HPLC and statistical analysis was performed. Results: Caffeine levels were higher in the plasma compared to all the brain structures studied. Different brain regions displayed similar caffeine concentrations. For all brain regions, the maximal concentration levels of caffeine were reached in the first 40 minutes after caffeine administration.Conclusions: The results support previous studies that show similar caffeine concentration between cortex and striatum, but also extend the results to other brain structures. Furthermore, caffeine concentration increases similarly in the plasma and brain structures. 40, 60 and 80 minutes after administration, caffeine concentration in the blood is almost two times higher than in the brain. This suggests that the effects of caffeine on different brain functions do not depend on pharmacokinetic differences between brain areas and are rather explained by pharmacodynamics. |
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AbstractList | Caffeine is a highly consumed stimulant of the nervous system. Although caffeine has diverse effects on different brain functions, little is known about the specific pharmacokinetics of this substance in the brain. For instance, most studies that assessed caffeine distribution in the rat brain have only measured caffeine levels in the cortex and striatum but not in more specific brain areas. Aims: The purpose of this work was to measure the caffeine concentration in blood and different brain regions (i.e. cortex, striatum, hippocampus, cerebellum and brainstem) at different times after the administration of a single intraperitoneal dose of caffeine. Methods: Adult Wistar rats (250 to 300 gr) were injected with a single intraperitoneal dose of 30 mg/Kg of caffeine. 20, 40, 60 and 80 minutes after administration, subjects were sacrificed by decapitation and samples of plasma, cerebral cortex, striatum, hippocampus, cerebellum and brainstem were obtained. Caffeine levels in the blood and each brain structure were measured by RP-HPLC and statistical analysis was performed. Results: Caffeine levels were higher in the plasma compared to all the brain structures studied. Different brain regions displayed similar caffeine concentrations. For all brain regions, the maximal concentration levels of caffeine were reached in the first 40 minutes after caffeine administration. Conclusions: The results support previous studies that show similar caffeine concentration between cortex and striatum, but also extend the results to other brain structures. Furthermore, caffeine concentration increases similarly in the plasma and brain structures. 40, 60 and 80 minutes after administration, caffeine concentration in the blood is almost two times higher than in the brain. This suggests that the effects of caffeine on different brain functions do not depend on pharmacokinetic differences between brain areas and are rather explained by pharmacodynamics. |
Author | Osorio-Forero, Alejandro Arias, Juan Miguel Restrepo, Francia Jiménez-Rodriguez, Alejandro Tamayo-Orrego, Lukas Orrego Cardozo, Mary |
AuthorAffiliation | Universidad Autónoma de Manizales |
AuthorAffiliation_xml | – sequence: 0 name: Universidad Autónoma de Manizales |
Author_xml | – sequence: 1 givenname: Mary surname: Orrego Cardozo fullname: Orrego Cardozo, Mary – sequence: 2 givenname: Alejandro surname: Jiménez-Rodriguez fullname: Jiménez-Rodriguez, Alejandro – sequence: 3 givenname: Alejandro surname: Osorio-Forero fullname: Osorio-Forero, Alejandro – sequence: 4 givenname: Francia surname: Restrepo fullname: Restrepo, Francia – sequence: 5 givenname: Juan Miguel surname: Arias fullname: Arias, Juan Miguel – sequence: 6 givenname: Lukas surname: Tamayo-Orrego fullname: Tamayo-Orrego, Lukas |
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CitedBy_id | crossref_primary_10_1016_j_bbr_2022_113982 crossref_primary_10_3389_fcell_2023_1072629 crossref_primary_10_1016_j_phyplu_2022_100391 |
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DocumentTitleAlternate | ESTRUCTURAS ENCEFÁLICAS DISTINTAS MUESTRAN CONCENTRACIÓN DE CAFEÍNA SIMILAR DESPUÉS DE LA ADMINISTRACIÓN DE UNA DOSIS ÚNICA |
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Keywords | farmacodinámica ebrain caffeine pharmacokinetics encéfalo plasma cafeína HPLC |
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Title | Different brain structures exhibit the same caffeine levels after the administration of a single dose of caffeine |
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