Molecular epidemiology and characterization of Streptococcus mutans strains in Korea
Objectives: We investigated the characteristics of Streptococcus mutans in the national culture collection from Korea. Twenty-nine (dental plaque, n=27; endodontic infections, n=1; blood, n=1)isolates were included in this study. Methods: Antimicrobial susceptibilities were tested using the disk dif...
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Published in | Journal of Korean Academy of Oral Health Vol. 44; no. 1; pp. 34 - 40 |
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Main Authors | , , , , |
Format | Journal Article |
Language | English |
Published |
대한예방치과·구강보건학회
01.03.2020
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Subjects | |
Online Access | Get full text |
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Summary: | Objectives: We investigated the characteristics of Streptococcus mutans in the national culture collection from Korea. Twenty-nine (dental plaque, n=27; endodontic infections, n=1; blood, n=1)isolates were included in this study.
Methods: Antimicrobial susceptibilities were tested using the disk diffusion test. Multilocus sequence typing (MLST), serotyping, and collagen-binding genes were used for polymerase chainreaction (PCR) and direct sequencing. A collagen-binding (to assess the adhesion properties) assay was performed. S. mutans demonstrated high susceptibility to antimicrobial agents. Differences in collagen-binding abilities of the cnm-positive and -negative groups were compared using the Mann-Whitney U test (P<0.05).
Results: MLST analyses revealed 25 sequence types (STs), 17 of which (ST213-ST229) contained new alleles. The strains were classified into four serotypes with the c type encompassing 79.3% of all strains, while the e , f , and k types representing 6.9% each. Analysis of the cnm and cbm genes, which encode the two surface adhesin components of S. mutans , revealed three cnm -positive strains, each displaying greater adhesion ability than those of the cnm -negative strains.
Conclusions: This study highlights the presence of a wide variety of S. mutans genotypes in Korea.
These findings may provide useful information regarding the pathogenesis of infectious diseases, such as dental caries. KCI Citation Count: 0 |
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Bibliography: | http://pdf.medrang.co.kr/Jkaoh/2020/044/Jkaoh044-01-07.pdf |
ISSN: | 1225-388X 2093-7784 |
DOI: | 10.11149/jkaoh.2020.44.1.34 |