Laboratory diagnosis of herpes simplex
Isolation and identification of HSV types are becoming increasingly important for oral and genital infections and are necessary for effective management of HSV encephalitis, neonatal infection, and infections in immunosuppressed patients. Herpes viruses are the most frequently isolated viruses in mo...
Saved in:
| Published in | Clinical microbiology newsletter Vol. 5; no. 4; pp. 21 - 23 |
|---|---|
| Main Author | |
| Format | Journal Article |
| Language | English |
| Published |
Elsevier Inc
15.02.1983
|
| Online Access | Get full text |
Cover
Loading…
| Abstract | Isolation and identification of HSV types are becoming increasingly important for oral and genital infections and are necessary for effective management of HSV encephalitis, neonatal infection, and infections in immunosuppressed patients. Herpes viruses are the most frequently isolated viruses in most clinical virology laboratories. Therefore the use of RK cells is recommended for several reasons: 1) they are commercially available and can be maintained for 2 to 3 weeks; 2) they will decrease the incidence of false-negative results which may exceed 15%; 3) they will decrease the time to virus isolation and the reporting of results; and 4) laboratory time to virus identification will be lessened because HSV strains are the only human viruses that will cause the characteristic rounding and rapid development of cytopathic effects typical of herpes viruses in RK cells. The two easiest methods for typing HSV isolates are the selective cell culture system and the BVDU sensitivity assay. Clear cut results are obtained with either system. |
|---|---|
| AbstractList | Isolation and identification of HSV types are becoming increasingly important for oral and genital infections and are necessary for effective management of HSV encephalitis, neonatal infection, and infections in immunosuppressed patients. Herpes viruses are the most frequently isolated viruses in most clinical virology laboratories. Therefore the use of RK cells is recommended for several reasons: 1) they are commercially available and can be maintained for 2 to 3 weeks; 2) they will decrease the incidence of false-negative results which may exceed 15%; 3) they will decrease the time to virus isolation and the reporting of results; and 4) laboratory time to virus identification will be lessened because HSV strains are the only human viruses that will cause the characteristic rounding and rapid development of cytopathic effects typical of herpes viruses in RK cells. The two easiest methods for typing HSV isolates are the selective cell culture system and the BVDU sensitivity assay. Clear cut results are obtained with either system. |
| Author | Mayo, Donald R. |
| Author_xml | – sequence: 1 givenname: Donald R. surname: Mayo fullname: Mayo, Donald R. organization: Virology Laboratory Veterans Administration Medical Center West Haven, Connecticut 06516, USA |
| BookMark | eNqFj01LxDAYhIOsYHf1Jwg9iR6qeZM0HyeRZf2Aggf1HNr0rUZ2myUp4v57213x6mlgmBnmmZNZH3ok5BzoNVCQNy8UjCwEN-ZS8ytNR7MwRyQDrfhkw4xkf5ETMk_pk1IqJYiMXFR1E2I9hLjLW1-_9yH5lIcu_8C4xZQnv9mu8fuUHHf1OuHZry7I2_3qdflYVM8PT8u7qnCg2FDUvGQMoJUUG2QNKClQghNgOgbKyU4xbBkrNTRKoDBUSy6l0WVXUqe44gtSHnZdDClF7Ow2-k0ddxaonWDtHtZOJFZzu4e1ZuzdHno4nvvyGG1yHnuHrY_oBtsG_8_CDxsmW8k |
| Cites_doi | 10.1128/JCM.15.4.733-736.1982 10.1093/infdis/145.6.829 10.1128/JCM.17.3.396-399.1983 10.1056/NEJM198107093050202 10.1056/NEJM198108063050604 10.1128/JCM.15.5.842-847.1982 10.1128/JCM.13.5.913-918.1981 10.1093/infdis/141.5.563 10.4049/jimmunol.104.2.289 10.1542/peds.66.4.495 10.1080/21548331.1979.11707470 10.1056/NEJM198102053040602 10.1542/peds.66.4.489 10.1056/NEJM197708112970601 |
| ContentType | Journal Article |
| Copyright | 1983 Elsevier Science Publishing Co., Inc. |
| Copyright_xml | – notice: 1983 Elsevier Science Publishing Co., Inc. |
| DBID | AAYXX CITATION |
| DOI | 10.1016/S0196-4399(83)80101-9 |
| DatabaseName | CrossRef |
| DatabaseTitle | CrossRef |
| DatabaseTitleList | |
| DeliveryMethod | fulltext_linktorsrc |
| Discipline | Biology |
| EISSN | 1873-4391 |
| EndPage | 23 |
| ExternalDocumentID | 10_1016_S0196_4399_83_80101_9 S0196439983801019 |
| GroupedDBID | --- --K --M .1- .FO .~1 0R~ 1B1 1P~ 1RT 1~. 1~5 29B 4.4 457 4G. 53G 5GY 5VS 7-5 71M 8P~ 9JM AAAJQ AACTN AAEDT AAEDW AAIAV AAIKJ AAKOC AALRI AAOAW AAQFI AAQXK AARKO AAXUO ABBQC ABFNM ABLVK ABMAC ABMZM ABXDB ABYKQ ACDAQ ACGFS ACIUM ACRLP ADBBV ADEZE ADMUD AEBSH AEKER AEVXI AFCTW AFKWA AFRHN AFTJW AFXIZ AGEKW AGHFR AGUBO AGYEJ AHHHB AIEXJ AIKHN AITUG AJBFU AJOXV AJRQY AJUYK ALMA_UNASSIGNED_HOLDINGS AMFUW AMRAJ ANZVX ASPBG AVWKF AXJTR AZFZN BKOJK BLXMC BNPGV CJTIS CNWQP CS3 DU5 EBS EFJIC EFLBG EJD EO8 EO9 EP2 EP3 FDB FEDTE FGOYB FIRID FNPLU FYGXN G-2 G-Q GBLVA HEJ HMG HMK HMO HVGLF HZ~ IHE J1W KOM LCYCR LUGTX M29 M41 MO0 N9A O-L O9- OAUVE OD- OO. OZT P-8 P-9 PC. Q38 R2- RIG ROL RPZ SAE SDF SDG SDP SEL SES SEW SIN SPCBC SSH SSI SSZ T5K UHS WUQ YYQ Z5R ZA5 ZUP ~G- AAXKI AAYXX AFJKZ AKRWK CITATION |
| ID | FETCH-LOGICAL-c172t-a352211d60ebe2b1764e61c419f217c6f72ed22581b74e49086366985f50c7373 |
| ISSN | 0196-4399 |
| IngestDate | Thu Sep 26 18:17:45 EDT 2024 Fri Feb 23 02:14:05 EST 2024 |
| IsPeerReviewed | true |
| IsScholarly | true |
| Issue | 4 |
| Language | English |
| LinkModel | OpenURL |
| MergedId | FETCHMERGED-LOGICAL-c172t-a352211d60ebe2b1764e61c419f217c6f72ed22581b74e49086366985f50c7373 |
| PageCount | 3 |
| ParticipantIDs | crossref_primary_10_1016_S0196_4399_83_80101_9 elsevier_sciencedirect_doi_10_1016_S0196_4399_83_80101_9 |
| PublicationCentury | 1900 |
| PublicationDate | 1983-02-15 |
| PublicationDateYYYYMMDD | 1983-02-15 |
| PublicationDate_xml | – month: 02 year: 1983 text: 1983-02-15 day: 15 |
| PublicationDecade | 1980 |
| PublicationTitle | Clinical microbiology newsletter |
| PublicationYear | 1983 |
| Publisher | Elsevier Inc |
| Publisher_xml | – name: Elsevier Inc |
| References | Landry (bib3) 1982; 15 Mayo (bib4) 1982; 15 Zheng (bib15) 1983; 17 Douglas, Couch (bib2) 1970; 104 De Clercq (bib1) 1980; 141 Reeves (bib8) 1981; 305 Roizman, Buchman (bib9) 1979; 14 Whitley (bib14) 1981; 304 Moseley (bib5) 1981; 13 Nordlund (bib7) 1977; 155 Saral (bib10) 1981; 305 Nahmias (bib6) 1982; 145 Whitley (bib13) 1977; 297 Whitley (bib11) 1980; 66 Whitley (bib12) 1980; 66 Reeves (10.1016/S0196-4399(83)80101-9_bib8) 1981; 305 Zheng (10.1016/S0196-4399(83)80101-9_bib15) 1983; 17 Whitley (10.1016/S0196-4399(83)80101-9_bib12) 1980; 66 Whitley (10.1016/S0196-4399(83)80101-9_bib13) 1977; 297 Nordlund (10.1016/S0196-4399(83)80101-9_bib7) 1977; 155 Landry (10.1016/S0196-4399(83)80101-9_bib3) 1982; 15 Whitley (10.1016/S0196-4399(83)80101-9_bib14) 1981; 304 Mayo (10.1016/S0196-4399(83)80101-9_bib4) 1982; 15 Douglas (10.1016/S0196-4399(83)80101-9_bib2) 1970; 104 De Clercq (10.1016/S0196-4399(83)80101-9_bib1) 1980; 141 Nahmias (10.1016/S0196-4399(83)80101-9_bib6) 1982; 145 Whitley (10.1016/S0196-4399(83)80101-9_bib11) 1980; 66 Roizman (10.1016/S0196-4399(83)80101-9_bib9) 1979; 14 Saral (10.1016/S0196-4399(83)80101-9_bib10) 1981; 305 Moseley (10.1016/S0196-4399(83)80101-9_bib5) 1981; 13 |
| References_xml | – volume: 304 start-page: 313 year: 1981 end-page: 318 ident: bib14 article-title: Herpes simplex encephalitis: vidarabine therapy and diagnostic problems publication-title: N. Engl. J. Med. contributor: fullname: Whitley – volume: 155 start-page: 118 year: 1977 end-page: 123 ident: bib7 article-title: The use of temperature sensitivity and selective cell culture systems for differentiation of herpes simplex virus types 1 and 2 in a clinical laboratory publication-title: Proc. Soc. Exp. Biol. Med. contributor: fullname: Nordlund – volume: 13 start-page: 913 year: 1981 end-page: 918 ident: bib5 article-title: Comparison of viral isolation, direct immunofluorescence, and indirect immunoperoxidase techniques for detection of genital publication-title: J. Clin. Microbiol. contributor: fullname: Moseley – volume: 305 start-page: 63 year: 1981 end-page: 67 ident: bib10 article-title: Acyclovir prophylaxis of herpes simplex virus infections publication-title: N. Engl. J. Med. contributor: fullname: Saral – volume: 15 start-page: 733 year: 1982 end-page: 736 ident: bib4 article-title: Differentiation of herpes simplex virus types 1 and 2 by sensitivity to (E)-5-(2-bromovinyl)-2′-deoxyuridine publication-title: J. Clin. Microbiol. contributor: fullname: Mayo – volume: 66 start-page: 489 year: 1980 end-page: 494 ident: bib12 article-title: The natural history of herpes simplex virus infection of mother and newborn publication-title: Pediatrics contributor: fullname: Whitley – volume: 15 start-page: 842 year: 1982 end-page: 847 ident: bib3 article-title: Comparison of guinea pig embryo cells, rabbit kidney cells and human embryonic lung fibroblast cell strains for isolation of herpes simplex virus publication-title: J. Clin. Microbiol. contributor: fullname: Landry – volume: 14 start-page: 95 year: 1979 end-page: 104 ident: bib9 article-title: The molecular epidemiology of herpes simplex viruses publication-title: Hosp. Pract. contributor: fullname: Buchman – volume: 66 start-page: 495 year: 1980 end-page: 501 ident: bib11 article-title: Vidarabine therapy of neonatal herpes simplex virus infection publication-title: Pediatrics contributor: fullname: Whitley – volume: 145 start-page: 829 year: 1982 end-page: 836 ident: bib6 article-title: Herpes simplex encephalitis: laboratory evaluations and their diagnostic significance publication-title: J. Infect. Dis. contributor: fullname: Nahmias – volume: 17 year: 1983 ident: bib15 article-title: Comparison of biological, biochemical, immunological and immunochemical techniques for typing of herpes simplex virus isolates publication-title: J. Clin. Microbiol. contributor: fullname: Zheng – volume: 297 start-page: 289 year: 1977 end-page: 294 ident: bib13 article-title: Adenine arabinoside therapy of biopsy-proven herpes simplex encephalitis publication-title: N. Engl. J. Med. contributor: fullname: Whitley – volume: 141 start-page: 563 year: 1980 end-page: 574 ident: bib1 article-title: Comparative efficacy of antiherpes drugs against different strains of herpes simplex virus publication-title: J. Infect. Dis. contributor: fullname: De Clercq – volume: 104 start-page: 289 year: 1970 end-page: 295 ident: bib2 article-title: A prospective study of chronic herpes simplex infection and recurrent herpes labialis in humans publication-title: J. Immunol. contributor: fullname: Couch – volume: 305 start-page: 315 year: 1981 end-page: 319 ident: bib8 article-title: Risk of recurrence after first episodes of genital herpes publication-title: N. Eng. J. Med. contributor: fullname: Reeves – volume: 15 start-page: 733 year: 1982 ident: 10.1016/S0196-4399(83)80101-9_bib4 article-title: Differentiation of herpes simplex virus types 1 and 2 by sensitivity to (E)-5-(2-bromovinyl)-2′-deoxyuridine publication-title: J. Clin. Microbiol. doi: 10.1128/JCM.15.4.733-736.1982 contributor: fullname: Mayo – volume: 145 start-page: 829 year: 1982 ident: 10.1016/S0196-4399(83)80101-9_bib6 article-title: Herpes simplex encephalitis: laboratory evaluations and their diagnostic significance publication-title: J. Infect. Dis. doi: 10.1093/infdis/145.6.829 contributor: fullname: Nahmias – volume: 17 year: 1983 ident: 10.1016/S0196-4399(83)80101-9_bib15 article-title: Comparison of biological, biochemical, immunological and immunochemical techniques for typing of herpes simplex virus isolates publication-title: J. Clin. Microbiol. doi: 10.1128/JCM.17.3.396-399.1983 contributor: fullname: Zheng – volume: 305 start-page: 63 year: 1981 ident: 10.1016/S0196-4399(83)80101-9_bib10 article-title: Acyclovir prophylaxis of herpes simplex virus infections publication-title: N. Engl. J. Med. doi: 10.1056/NEJM198107093050202 contributor: fullname: Saral – volume: 305 start-page: 315 year: 1981 ident: 10.1016/S0196-4399(83)80101-9_bib8 article-title: Risk of recurrence after first episodes of genital herpes publication-title: N. Eng. J. Med. doi: 10.1056/NEJM198108063050604 contributor: fullname: Reeves – volume: 15 start-page: 842 year: 1982 ident: 10.1016/S0196-4399(83)80101-9_bib3 article-title: Comparison of guinea pig embryo cells, rabbit kidney cells and human embryonic lung fibroblast cell strains for isolation of herpes simplex virus publication-title: J. Clin. Microbiol. doi: 10.1128/JCM.15.5.842-847.1982 contributor: fullname: Landry – volume: 13 start-page: 913 year: 1981 ident: 10.1016/S0196-4399(83)80101-9_bib5 article-title: Comparison of viral isolation, direct immunofluorescence, and indirect immunoperoxidase techniques for detection of genital Herpes simplex virus infection publication-title: J. Clin. Microbiol. doi: 10.1128/JCM.13.5.913-918.1981 contributor: fullname: Moseley – volume: 155 start-page: 118 year: 1977 ident: 10.1016/S0196-4399(83)80101-9_bib7 article-title: The use of temperature sensitivity and selective cell culture systems for differentiation of herpes simplex virus types 1 and 2 in a clinical laboratory contributor: fullname: Nordlund – volume: 141 start-page: 563 year: 1980 ident: 10.1016/S0196-4399(83)80101-9_bib1 article-title: Comparative efficacy of antiherpes drugs against different strains of herpes simplex virus publication-title: J. Infect. Dis. doi: 10.1093/infdis/141.5.563 contributor: fullname: De Clercq – volume: 104 start-page: 289 year: 1970 ident: 10.1016/S0196-4399(83)80101-9_bib2 article-title: A prospective study of chronic herpes simplex infection and recurrent herpes labialis in humans publication-title: J. Immunol. doi: 10.4049/jimmunol.104.2.289 contributor: fullname: Douglas – volume: 66 start-page: 495 year: 1980 ident: 10.1016/S0196-4399(83)80101-9_bib11 article-title: Vidarabine therapy of neonatal herpes simplex virus infection publication-title: Pediatrics doi: 10.1542/peds.66.4.495 contributor: fullname: Whitley – volume: 14 start-page: 95 year: 1979 ident: 10.1016/S0196-4399(83)80101-9_bib9 article-title: The molecular epidemiology of herpes simplex viruses publication-title: Hosp. Pract. doi: 10.1080/21548331.1979.11707470 contributor: fullname: Roizman – volume: 304 start-page: 313 year: 1981 ident: 10.1016/S0196-4399(83)80101-9_bib14 article-title: Herpes simplex encephalitis: vidarabine therapy and diagnostic problems publication-title: N. Engl. J. Med. doi: 10.1056/NEJM198102053040602 contributor: fullname: Whitley – volume: 66 start-page: 489 year: 1980 ident: 10.1016/S0196-4399(83)80101-9_bib12 article-title: The natural history of herpes simplex virus infection of mother and newborn publication-title: Pediatrics doi: 10.1542/peds.66.4.489 contributor: fullname: Whitley – volume: 297 start-page: 289 year: 1977 ident: 10.1016/S0196-4399(83)80101-9_bib13 article-title: Adenine arabinoside therapy of biopsy-proven herpes simplex encephalitis publication-title: N. Engl. J. Med. doi: 10.1056/NEJM197708112970601 contributor: fullname: Whitley |
| SSID | ssj0006614 |
| Score | 1.2545768 |
| Snippet | Isolation and identification of HSV types are becoming increasingly important for oral and genital infections and are necessary for effective management of HSV... |
| SourceID | crossref elsevier |
| SourceType | Aggregation Database Publisher |
| StartPage | 21 |
| Title | Laboratory diagnosis of herpes simplex |
| URI | https://dx.doi.org/10.1016/S0196-4399(83)80101-9 |
| Volume | 5 |
| hasFullText | 1 |
| inHoldings | 1 |
| isFullTextHit | |
| isPrint | |
| link | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV1bS8MwFA46EXwRrzhv9EFFkcxe0jR5HEMRFR_mxL2VJk1goNtwFZy_3pOlNy-I-hJKIJfma0--nOQ7QehAExEmqauxK1KCieYMJ6lQWAUy1DShHEqZ0xa39PKeXPXDfnUr4ExdkomWfPtWV_IfVCEPcDUq2T8gW1YKGfAM-EIKCEP6K4xvLIJmmzy1R-ZsdBHAYawmp5OBCf37WuefnUII-TSoRWAy1Pqx0PXkDurpyPJr4zo-7bYq74DHmdmTxVYfWTgMOcVmzVG3eGENWPLBetXmQSsD_mJh7WL_rqz30FxXfOhzZoLVYV5NK8VW-qfZpjwDWDtexmlsqopZEM-qifk8WvDBcoDJWmhfdx-uy8nV0AmrgLfNV6Kss6pPxyw4yfvzPd2oUYjeClrOub_TtkCuojk1XEOL9jbQ6To6quB0SjidkXYsnE4O5wa6vzjvdS5xfo0FlsAOM5wYjut5KXXhh_GFF1GiqCeJxzWsByXVka9SMKuwgIiIMhuxNKCUs1CHroyCKNhEjeFoqLaQEyXS48rVrtAJYURx4SY0SaWvmDAK5SZqFW8bj220kvjHcW4iVoxJnFMuS6ViwPvnott_bWsHLVVf6C5qZM8vag8YXSb2c5TfAeS1QzM |
| link.rule.ids | 315,783,787,27936,27937 |
| linkProvider | Library Specific Holdings |
| openUrl | ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Laboratory+diagnosis+of+herpes+simplex&rft.jtitle=Clinical+microbiology+newsletter&rft.au=Mayo%2C+Donald+R.&rft.date=1983-02-15&rft.issn=0196-4399&rft.volume=5&rft.issue=4&rft.spage=21&rft.epage=23&rft_id=info:doi/10.1016%2FS0196-4399%2883%2980101-9&rft.externalDBID=n%2Fa&rft.externalDocID=10_1016_S0196_4399_83_80101_9 |
| thumbnail_l | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=0196-4399&client=summon |
| thumbnail_m | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=0196-4399&client=summon |
| thumbnail_s | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=0196-4399&client=summon |