Pigeon Liver Malic Enzyme
Pigeon liver malic enzyme (malate dehydrogenase (decarboxylating), EC 1.1.1.38) was purified and crystallized from ammonium sulfate solution as its triphosphopyridine nucleotide complex. The crystalline protein is homogeneous in the ultracentrifuge, in gradient centrifugation, and in gradient chroma...
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| Published in | The Journal of biological chemistry Vol. 242; no. 3; pp. 520 - 526 |
|---|---|
| Main Authors | , |
| Format | Journal Article |
| Language | English |
| Published |
American Society for Biochemistry and Molecular Biology
10.02.1967
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| Online Access | Get full text |
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| Abstract | Pigeon liver malic enzyme (malate dehydrogenase (decarboxylating), EC 1.1.1.38) was purified and crystallized from ammonium
sulfate solution as its triphosphopyridine nucleotide complex. The crystalline protein is homogeneous in the ultracentrifuge,
in gradient centrifugation, and in gradient chromatography. A constant specific activity of 28 units per mg of protein is
obtained. The crystalline enzyme has an s 20 of 10.0, an apparent diffusion coefficient of 3.17 x 10 -7 cm 2 per sec, and an apparent partial specific volume of 0.74. Its molecular weight is 2.8 x 10 5 . Purified malic enzyme has an ultraviolet absorption maximum at 278 mµ. The extinction coefficients for the purified enzyme
and the crystalline TPN complex are 0.86 and 0.92, respectively, for a 0.10% protein solution. The purified enzyme catalyzes
the decarboxylation of oxalacetate at a rate comparable to the decarboxylation of malate. Reversible inactivation of these
activities occurs upon storage of the enzyme. Reactivation is obtained by incubation with dithiothreitol. |
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| AbstractList | Pigeon liver malic enzyme (malate dehydrogenase (decarboxylating), EC 1.1.1.38) was purified and crystallized from ammonium
sulfate solution as its triphosphopyridine nucleotide complex. The crystalline protein is homogeneous in the ultracentrifuge,
in gradient centrifugation, and in gradient chromatography. A constant specific activity of 28 units per mg of protein is
obtained. The crystalline enzyme has an s 20 of 10.0, an apparent diffusion coefficient of 3.17 x 10 -7 cm 2 per sec, and an apparent partial specific volume of 0.74. Its molecular weight is 2.8 x 10 5 . Purified malic enzyme has an ultraviolet absorption maximum at 278 mµ. The extinction coefficients for the purified enzyme
and the crystalline TPN complex are 0.86 and 0.92, respectively, for a 0.10% protein solution. The purified enzyme catalyzes
the decarboxylation of oxalacetate at a rate comparable to the decarboxylation of malate. Reversible inactivation of these
activities occurs upon storage of the enzyme. Reactivation is obtained by incubation with dithiothreitol. |
| Author | Robert Y. Hsu Henry A. Lardy |
| Author_xml | – sequence: 1 givenname: Robert Y. surname: Hsu fullname: Hsu, Robert Y. – sequence: 2 givenname: Henry A. surname: Lardy fullname: Lardy, Henry A. |
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| Cites_doi | 10.1016/S0021-9258(18)56233-5 10.1016/0006-3002(62)91168-X 10.1016/S0021-9258(18)65700-X 10.1016/S0021-9258(18)56232-3 10.1016/S0021-9258(18)64768-4 10.1042/bj0530436 10.1016/S0021-9258(18)43126-2 10.1111/j.1749-6632.1959.tb44173.x 10.1016/S0021-9258(18)96305-2 10.1016/S0021-9258(18)48645-0 10.1016/S0021-9258(18)57021-6 10.1073/pnas.52.5.1255 10.1016/S0021-9258(18)57307-5 10.1016/S0021-9258(18)64180-8 10.1021/bi00899a015 10.1016/S0021-9258(17)30974-2 10.1016/S0021-9258(17)34969-4 10.1016/S0065-2571(64)80004-2 |
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| References | Gornall (10.1016/S0021-9258(18)96304-0_bib19) 1949; 177 Blanchard (10.1016/S0021-9258(18)96304-0_bib8) 1950; 187 Utter (10.1016/S0021-9258(18)96304-0_bib13) 1954; 207 Kraemer (10.1016/S0021-9258(18)96304-0_bib18) 1940 Lwoff (10.1016/S0021-9258(18)96304-0_bib4) 1947; 224 Shrago (10.1016/S0021-9258(18)96304-0_bib14) 1963; 238 Wise (10.1016/S0021-9258(18)96304-0_bib17) 1964; 52 Heyningen (10.1016/S0021-9258(18)96304-0_bib7) 1953; 53 Ochoa (10.1016/S0021-9258(18)96304-0_bib6) 1947; 170 Lardy (10.1016/S0021-9258(18)96304-0_bib15) 1964; 2 Ochoa (10.1016/S0021-9258(18)96304-0_bib23) 1948; 174 Racker (10.1016/S0021-9258(18)96304-0_bib24) 1955; I Utter (10.1016/S0021-9258(18)96304-0_bib11) 1959; 72 Lowry (10.1016/S0021-9258(18)96304-0_bib20) 1957; III Lwoff (10.1016/S0021-9258(18)96304-0_bib3) 1947; 224 Ochoa (10.1016/S0021-9258(18)96304-0_bib5) 1947; 167 Kun (10.1016/S0021-9258(18)96304-0_bib9) 1963; 7 Krebs (10.1016/S0021-9258(18)96304-0_bib10) 1954; 95 Young (10.1016/S0021-9258(18)96304-0_bib16) 1964; 3 Noltman (10.1016/S0021-9258(18)96304-0_bib26) 1963; 7 Moulder (10.1016/S0021-9258(18)96304-0_bib2) 1945; 160 Ochoa (10.1016/S0021-9258(18)96304-0_bib12) 1950; 187 Schachman (10.1016/S0021-9258(18)96304-0_bib22) 1957; IV Hsu (10.1016/S0021-9258(18)96304-0_bib27) 1967; 242 Fitch (10.1016/S0021-9258(18)96304-0_bib25) 1962; 57 Martin (10.1016/S0021-9258(18)96304-0_bib21) 1961; 236 Rutter (10.1016/S0021-9258(18)96304-0_bib1) 1958; 233 |
| References_xml | – volume: 187 start-page: 875 year: 1950 ident: 10.1016/S0021-9258(18)96304-0_bib8 publication-title: J. Biol. Chem. doi: 10.1016/S0021-9258(18)56233-5 contributor: fullname: Blanchard – volume: 57 start-page: 588 year: 1962 ident: 10.1016/S0021-9258(18)96304-0_bib25 publication-title: Biochim. Biophys. Acta doi: 10.1016/0006-3002(62)91168-X contributor: fullname: Fitch – volume: 207 start-page: 821 year: 1954 ident: 10.1016/S0021-9258(18)96304-0_bib13 publication-title: J. Biol. Chem. doi: 10.1016/S0021-9258(18)65700-X contributor: fullname: Utter – volume: 7 start-page: 223 year: 1963 ident: 10.1016/S0021-9258(18)96304-0_bib26 contributor: fullname: Noltman – volume: 7 start-page: 149 year: 1963 ident: 10.1016/S0021-9258(18)96304-0_bib9 contributor: fullname: Kun – volume: 187 start-page: 863 year: 1950 ident: 10.1016/S0021-9258(18)96304-0_bib12 publication-title: J. Biol. Chem. doi: 10.1016/S0021-9258(18)56232-3 contributor: fullname: Ochoa – volume: 224 start-page: 303 year: 1947 ident: 10.1016/S0021-9258(18)96304-0_bib4 publication-title: Compt. Rend. contributor: fullname: Lwoff – volume: 233 start-page: 374 year: 1958 ident: 10.1016/S0021-9258(18)96304-0_bib1 publication-title: J. Biol. Chem. doi: 10.1016/S0021-9258(18)64768-4 contributor: fullname: Rutter – volume: 53 start-page: 436 year: 1953 ident: 10.1016/S0021-9258(18)96304-0_bib7 publication-title: Biochem. J. doi: 10.1042/bj0530436 contributor: fullname: Heyningen – volume: 160 start-page: 305 year: 1945 ident: 10.1016/S0021-9258(18)96304-0_bib2 publication-title: J. Biol. Chem. doi: 10.1016/S0021-9258(18)43126-2 contributor: fullname: Moulder – volume: 72 start-page: 451 year: 1959 ident: 10.1016/S0021-9258(18)96304-0_bib11 publication-title: Ann. N. Y. Acad. Sci. doi: 10.1111/j.1749-6632.1959.tb44173.x contributor: fullname: Utter – volume: IV start-page: 32 year: 1957 ident: 10.1016/S0021-9258(18)96304-0_bib22 contributor: fullname: Schachman – volume: III start-page: 448 year: 1957 ident: 10.1016/S0021-9258(18)96304-0_bib20 contributor: fullname: Lowry – volume: 242 start-page: 527 year: 1967 ident: 10.1016/S0021-9258(18)96304-0_bib27 publication-title: J. Biol. Chem. doi: 10.1016/S0021-9258(18)96305-2 contributor: fullname: Hsu – start-page: 59 year: 1940 ident: 10.1016/S0021-9258(18)96304-0_bib18 contributor: fullname: Kraemer – volume: 238 start-page: 3188 year: 1963 ident: 10.1016/S0021-9258(18)96304-0_bib14 publication-title: J. Biol. Chem. doi: 10.1016/S0021-9258(18)48645-0 contributor: fullname: Shrago – volume: 177 start-page: 751 year: 1949 ident: 10.1016/S0021-9258(18)96304-0_bib19 publication-title: J. Biol. Chem. doi: 10.1016/S0021-9258(18)57021-6 contributor: fullname: Gornall – volume: 52 start-page: 1255 year: 1964 ident: 10.1016/S0021-9258(18)96304-0_bib17 publication-title: Proc. Natl. Acad. Sci. U. S. doi: 10.1073/pnas.52.5.1255 contributor: fullname: Wise – volume: 174 start-page: 979 year: 1948 ident: 10.1016/S0021-9258(18)96304-0_bib23 publication-title: J. Biol. Chem. doi: 10.1016/S0021-9258(18)57307-5 contributor: fullname: Ochoa – volume: 236 start-page: 1372 year: 1961 ident: 10.1016/S0021-9258(18)96304-0_bib21 publication-title: J. Biol. Chem. doi: 10.1016/S0021-9258(18)64180-8 contributor: fullname: Martin – volume: 3 start-page: 1687 year: 1964 ident: 10.1016/S0021-9258(18)96304-0_bib16 publication-title: Biochemistry doi: 10.1021/bi00899a015 contributor: fullname: Young – volume: I start-page: 500 year: 1955 ident: 10.1016/S0021-9258(18)96304-0_bib24 contributor: fullname: Racker – volume: 224 start-page: 678 year: 1947 ident: 10.1016/S0021-9258(18)96304-0_bib3 publication-title: Compt. Rend. contributor: fullname: Lwoff – volume: 167 start-page: 871 year: 1947 ident: 10.1016/S0021-9258(18)96304-0_bib5 publication-title: J. Biol. Chem. doi: 10.1016/S0021-9258(17)30974-2 contributor: fullname: Ochoa – volume: 170 start-page: 413 year: 1947 ident: 10.1016/S0021-9258(18)96304-0_bib6 publication-title: J. Biol. Chem. doi: 10.1016/S0021-9258(17)34969-4 contributor: fullname: Ochoa – volume: 95 start-page: 19 year: 1954 ident: 10.1016/S0021-9258(18)96304-0_bib10 publication-title: Bull. Johns Hopkins Hosp. contributor: fullname: Krebs – volume: 2 start-page: 39 year: 1964 ident: 10.1016/S0021-9258(18)96304-0_bib15 publication-title: Advan. Enzyme Regulation doi: 10.1016/S0065-2571(64)80004-2 contributor: fullname: Lardy |
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