Versatile, cost-effective and highly-multiplex nucleic acid detection through the Engineered Hairpin Cleavage Amplification (EHCA) strategy

• Published in: Chemical engineering journal (Lausanne, Switzerland : 1996) Vol. 521; p. 166484
• Main Authors: Liu, Zhaocheng, Zhang, Rui, Zhang, Yi, Mu, Huijun, Jiang, Xinyi, Zou, Jian, Wang, Tingting, Yin, Ying
• Format: Journal Article
• Language: English
• Published: Elsevier B.V 01.10.2025
• Subjects: CRISPR/Cas12a; ddPCR; hairpin amplicon; melting curve analysis (MCA); multiplex amplification; ddPCR; multiplex amplification; hairpin amplicon; melting curve analysis (MCA); CRISPR/Cas12a

Real-time quantitative PCR (qPCR), droplet digital PCR (ddPCR), and CRISPR/Cas diagnostics typically rely on expensive, target-specific fluorescence probes or CRISPR RNA (crRNA) for precise nucleic acid detection, and their multiplexing capability is limited by the scarcity of fluorescent colors in...