TURMERIC EXTRACT POTENTIAL INHIBIT INFLAMMATORY MARKER IN LPS-STIMULATED MARCOPHAGE CELLS

• Published in: International journal of applied pharmaceutics pp. 7 - 11
• Main Authors: WIDOWATI, WAHYU, JASAPUTRA, DIANA KRISANTI, GUNAWAN, KAMILA YASHFA, KUSUMA, HANNA SARI WIDYA, ARUMWARDANA, SEILA, WAHYUNI, CINTANI DEWI, LISTER, I. NYOMAN EHRICH, GINTING, CHRISMIS NOVALINDA, GIRSANG, ERMI, RIZAL, RIZAL
• Format: Journal Article
• Language: English
• Published: 10.03.2021
• ISSN: 0975-7058; 0975-7058
• DOI: 10.22159/ijap.2021.v13s3.01

Objective: Inflammation can be induced by microbiological, chemical, physical factors and plays roles in inflammatory diseases. Turmeric (Curcuma longa L.) has been widely used to provide a diverse array of biological activities, including anti-inflammatory, antimicrobial, also antioxidant. The Turmeric extract (TE) anti-inflammatory potential was conducted using a Lipopolysaccharide (LPS)-induced RAW264.7 macrophage cell line by inhibiting inflammatory mediators especially IL-6, PGE-2, IL-1β, COX-2, TNF-α, iNOS, also NO level. Methods: The TE safe concentration in LPS-induced macrophage cell line was measured using MTS assay for further assay. The inflammatory markers (IL-6, PGE-2, COX-2, IL-1β, TNF-α, iNOS, NO) were measured using ELISA assay and NO by the nitrate/nitrite colorimetric assay in LPS-induced RAW264.7 cell line. LPS induced inflammatory marker by increasing inflammatory marker (IL-6, PGE-2, COX-2, IL-1β, TNF-α, iNOS, NO). Results: TE with 100 to 25 µg/ml, caused a significant reduction of cells viability, reaching only 30.27 % live cells. TE with lower concentrations (7.5; 5; 2.5 µg/ml) had no cytotoxic effect on macrophage cells (viability 117.31-131.08 %). LPS induction caused an increase in inflammatory cytokines IL-1β, PGE-2, IL-6, COX-2, TNF-α as well as iNOS and NO. Turmeric extract caused the reduction of the inflammatory cytokines in a dose-dependent manner. Conclusion: The research resulted that TE has anti-inflammatory activity by decreasing IL-6, PGE-2, COX-2, IL-1β, TNF-α, iNOS, and NO level on LPS-induced RAW264.7 cells.