Study on Semen Freezing Preservation of German Shepherd Dogs
In order to prolong semen preservation and improve pregnancy rate, semen freezing was studied with German shepherd dogs for experimental animals. The semen of four dogs was collected 40 times in four dilution frozen into two formulations, according to the sperm motility to compare the advantages and...
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| Published in | The journal of Northeast Agricultural University Vol. 19; no. 4; pp. 53 - 57 |
|---|---|
| Main Authors | , , , |
| Format | Journal Article |
| Language | English |
| Published |
Elsevier (Singapore) Pte Ltd
01.12.2012
College of Animal Sciences and Technology, Northeast Agricultural University, Harbin 150030, China |
| Subjects | |
| Online Access | Get full text |
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| Abstract | In order to prolong semen preservation and improve pregnancy rate, semen freezing was studied with German shepherd dogs for experimental animals. The semen of four dogs was collected 40 times in four dilution frozen into two formulations, according to the sperm motility to compare the advantages and disadvantages. The result indicated that the sperm motility of the pellet frozen semen in dilute 2 was significantly higher than that in dilution 1, 3, and 4 （P0.01）. The sperm motility of dogs semen with fried smoked method was notablely higher than that of frozen semen of program method （P0.01）. The dilution which contained yolk-Tris mainly was the best; the pellet semen frozen with fried smoked method was superior to tuble semen frozen with program freezing method; sperm motility of pellet semen was higher than that of tuble semen in the same dilution. The conception rate and litter size of the natural matting were higher than AI. |
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| AbstractList | In order to prolong semen preservation and improve pregnancy rate, semen freezing was studied with German shepherd dogs for experimental animals. The semen of four dogs was collected 40 times in four dilution frozen into two formulations, according to the sperm motility to compare the advantages and disadvantages. The result indicated that the sperm motility of the pellet frozen semen in dilute 2 was significantly higher than that in dilution 1, 3, and 4 （P0.01）. The sperm motility of dogs semen with fried smoked method was notablely higher than that of frozen semen of program method （P0.01）. The dilution which contained yolk-Tris mainly was the best; the pellet semen frozen with fried smoked method was superior to tuble semen frozen with program freezing method; sperm motility of pellet semen was higher than that of tuble semen in the same dilution. The conception rate and litter size of the natural matting were higher than AI. S829.2; In order to prolong semen preservation and improve pregnancy rate, semen freezing was studied with German shepherd dogs for experimental animals. The semen of four dogs was collected 40 times in four dilution frozen into two formulations, according to the sperm motility to compare the advantages and disadvantages. The result indicated that the sperm motility of the pellet frozen semen in dilute 2 was significantly higher than that in dilution 1, 3, and 4 (P0.01). The sperm motility of dogs semen with fried smoked method was notablely higher than that of frozen semen of program method (P0.01). The dilution which contained yolk-Tris mainly was the best; the pellet semen frozen with fried smoked method was superior to tuble semen frozen with program freezing method; sperm motility of pellet semen was higher than that of tuble semen in the same dilution. The conception rate and litter size of the natural matting were higher than AI. In order to prolong semen preservation and improve pregnancy rate, semen freezing was studied with German shepherd dogs for experimental animals. The semen of four dogs was collected 40 times in four dilution frozen into two formulations, according to the sperm motility to compare the advantages and disadvantages. The result indicated that the sperm motility of the pellet frozen semen in dilute 2 was significantly higher than that in dilution 1, 3, and 4 (P<0.01). The sperm motility of dogs semen with fried smoked method was notablely higher than that of frozen semen of program method (P<0.01). The dilution which contained yolk-Tris mainly was the best; the pellet semen frozen with fried smoked method was superior to tuble semen frozen with program freezing method; sperm motility of pellet semen was higher than that of tuble semen in the same dilution. The conception rate and litter size of the natural matting were higher than AI. |
| Author | Huang He Tian Ya-guang Zheng Peng Zhang Gui-xue |
| AuthorAffiliation | College of Animal Sciences and Technology, Northeast Agricultural University, Harbin 150030, China |
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| Cites_doi | 10.1530/jrf.0.0450189 10.1016/0093-691X(89)90550-5 |
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| Copyright | 2013 Asian Pacific Tropical Medicine Press Copyright © Wanfang Data Co. Ltd. All Rights Reserved. |
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| Keywords | dilution dog semen freezing fried smoked method |
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| Notes | dog; dilution; semen freezing; fried smoked method Huang He, Tian Ya-guang, Zheng Peng, and Zhang Gui-xue College of Animal Sciences and Technology, Northeast Agricultural University, Harbin 150030, China 23-1392/S In order to prolong semen preservation and improve pregnancy rate, semen freezing was studied with German shepherd dogs for experimental animals. The semen of four dogs was collected 40 times in four dilution frozen into two formulations, according to the sperm motility to compare the advantages and disadvantages. The result indicated that the sperm motility of the pellet frozen semen in dilute 2 was significantly higher than that in dilution 1, 3, and 4 （P0.01）. The sperm motility of dogs semen with fried smoked method was notablely higher than that of frozen semen of program method （P0.01）. The dilution which contained yolk-Tris mainly was the best; the pellet semen frozen with fried smoked method was superior to tuble semen frozen with program freezing method; sperm motility of pellet semen was higher than that of tuble semen in the same dilution. The conception rate and litter size of the natural matting were higher than AI. |
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| Publisher | Elsevier (Singapore) Pte Ltd College of Animal Sciences and Technology, Northeast Agricultural University, Harbin 150030, China |
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| References | Pan, Liu, Ma (bib4) 1986; 2 Zhao, Li, Sun (bib11) 2008; 39 Lian, Li (bib1) 1997; 4 Olar, Bowen, Pickett (bib3) 1989; 31 Seager (bib6) 1975; 45 Zhang (bib12) 2004 Yu, Pang, Li (bib10) 1997; 3 Wang, Liu, Zeng (bib8) 2008; 2 Wu (bib9) 1976; 22 Oettle (bib2) 1988; 59 Seager (bib5) 1969; 27 Wang, Yang, Zhou (bib7) 1991; 5 Yu (10.1016/S1006-8104(13)60051-3_bib10) 1997; 3 Zhao (10.1016/S1006-8104(13)60051-3_bib11) 2008; 39 Seager (10.1016/S1006-8104(13)60051-3_bib5) 1969; 27 Wang (10.1016/S1006-8104(13)60051-3_bib7) 1991; 5 Pan (10.1016/S1006-8104(13)60051-3_bib4) 1986; 2 Lian (10.1016/S1006-8104(13)60051-3_bib1) 1997; 4 Wang (10.1016/S1006-8104(13)60051-3_bib8) 2008; 2 Zhang (10.1016/S1006-8104(13)60051-3_bib12) 2004 Olar (10.1016/S1006-8104(13)60051-3_bib3) 1989; 31 Seager (10.1016/S1006-8104(13)60051-3_bib6) 1975; 45 Wu (10.1016/S1006-8104(13)60051-3_bib9) 1976; 22 Oettle (10.1016/S1006-8104(13)60051-3_bib2) 1988; 59 |
| References_xml | – volume: 59 start-page: 28 year: 1988 end-page: 70 ident: bib2 article-title: Sperm abnormalities in the dog. A light and electron-microscopic study publication-title: Vet Med Rev contributor: fullname: Oettle – volume: 22 start-page: 28 year: 1976 end-page: 32 ident: bib9 article-title: Artificial insemination technique of dog publication-title: Journal of Animal Reproduction contributor: fullname: Wu – volume: 39 start-page: 70 year: 2008 end-page: 75 ident: bib11 article-title: Study on the pellet freezing technique of boar semen publication-title: Journal of Northeast Agricultural University contributor: fullname: Sun – start-page: 221 year: 2004 ident: bib12 publication-title: Animal reproduction science contributor: fullname: Zhang – volume: 31 start-page: 451 year: 1989 ident: bib3 article-title: Influence of extender, cryoperservative and seminal processing procedures on postthaw motility of canine spermatozoa frozen in straws publication-title: Theriogenology contributor: fullname: Pickett – volume: 2 start-page: 105 year: 1986 end-page: 106 ident: bib4 article-title: Semen freezing technique of dog publication-title: Animal Husbandy And Veterinary Medicine contributor: fullname: Ma – volume: 3 start-page: 289 year: 1997 end-page: 291 ident: bib10 article-title: Study on freezing canine pellet semen by the new method of frying in liquid nitrogen publication-title: Chinese Journal of Veterinary Science contributor: fullname: Li – volume: 4 start-page: 52 year: 1997 end-page: 54 ident: bib1 article-title: Biological effects of deer semen on development of testes in mice publication-title: Journal of Northeast Agricultural University contributor: fullname: Li – volume: 5 start-page: 35 year: 1991 end-page: 36 ident: bib7 article-title: Study on semen freezing of dog and artificial insemination publication-title: Heilongjiang Animal Science And Veterinary Medicine contributor: fullname: Zhou – volume: 27 start-page: 6 year: 1969 ident: bib5 article-title: Storage of boar semen publication-title: AI Digest contributor: fullname: Seager – volume: 2 start-page: 12 year: 2008 end-page: 16 ident: bib8 article-title: Advance in international development of canine semen cryopreservation publication-title: Chinese Journal of Animal Science contributor: fullname: Zeng – volume: 45 start-page: 189 year: 1975 end-page: 192 ident: bib6 article-title: Relation of semen quality tosperm transport, fertilization and embryo quality in ruminants publication-title: Reprod J Fertil contributor: fullname: Seager – volume: 2 start-page: 12 issue: 15 year: 2008 ident: 10.1016/S1006-8104(13)60051-3_bib8 article-title: Advance in international development of canine semen cryopreservation publication-title: Chinese Journal of Animal Science contributor: fullname: Wang – volume: 45 start-page: 189 year: 1975 ident: 10.1016/S1006-8104(13)60051-3_bib6 article-title: Relation of semen quality tosperm transport, fertilization and embryo quality in ruminants publication-title: Reprod J Fertil doi: 10.1530/jrf.0.0450189 contributor: fullname: Seager – volume: 31 start-page: 451 issue: 2 year: 1989 ident: 10.1016/S1006-8104(13)60051-3_bib3 article-title: Influence of extender, cryoperservative and seminal processing procedures on postthaw motility of canine spermatozoa frozen in straws publication-title: Theriogenology doi: 10.1016/0093-691X(89)90550-5 contributor: fullname: Olar – volume: 5 start-page: 35 year: 1991 ident: 10.1016/S1006-8104(13)60051-3_bib7 article-title: Study on semen freezing of dog and artificial insemination publication-title: Heilongjiang Animal Science And Veterinary Medicine contributor: fullname: Wang – volume: 22 start-page: 28 issue: 1 year: 1976 ident: 10.1016/S1006-8104(13)60051-3_bib9 article-title: Artificial insemination technique of dog publication-title: Journal of Animal Reproduction contributor: fullname: Wu – volume: 59 start-page: 28 year: 1988 ident: 10.1016/S1006-8104(13)60051-3_bib2 article-title: Sperm abnormalities in the dog. A light and electron-microscopic study publication-title: Vet Med Rev contributor: fullname: Oettle – volume: 4 start-page: 52 issue: 1 year: 1997 ident: 10.1016/S1006-8104(13)60051-3_bib1 article-title: Biological effects of deer semen on development of testes in mice publication-title: Journal of Northeast Agricultural University contributor: fullname: Lian – volume: 39 start-page: 70 issue: 3 year: 2008 ident: 10.1016/S1006-8104(13)60051-3_bib11 article-title: Study on the pellet freezing technique of boar semen publication-title: Journal of Northeast Agricultural University contributor: fullname: Zhao – volume: 2 start-page: 105 issue: 3 year: 1986 ident: 10.1016/S1006-8104(13)60051-3_bib4 article-title: Semen freezing technique of dog publication-title: Animal Husbandy And Veterinary Medicine contributor: fullname: Pan – start-page: 221 year: 2004 ident: 10.1016/S1006-8104(13)60051-3_bib12 contributor: fullname: Zhang – volume: 27 start-page: 6 issue: 3 year: 1969 ident: 10.1016/S1006-8104(13)60051-3_bib5 article-title: Storage of boar semen publication-title: AI Digest contributor: fullname: Seager – volume: 3 start-page: 289 year: 1997 ident: 10.1016/S1006-8104(13)60051-3_bib10 article-title: Study on freezing canine pellet semen by the new method of frying in liquid nitrogen publication-title: Chinese Journal of Veterinary Science contributor: fullname: Yu |
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| Snippet | In order to prolong semen preservation and improve pregnancy rate, semen freezing was studied with German shepherd dogs for experimental animals. The semen of... S829.2; In order to prolong semen preservation and improve pregnancy rate, semen freezing was studied with German shepherd dogs for experimental animals. The... |
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| Title | Study on Semen Freezing Preservation of German Shepherd Dogs |
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