Evaluation of Simple and Rapid Shiga Toxin Gene Detection of Enterohemorrhagic Escherichia coliin Food Using 3MTM Molecular Detection Assay2-STEC Gene Screen Kit

The sensitivity of the 3M TM Molecular Detection Assay 2-STEC Gene Screen (stx) assay (3M MDA2 STEC assay) was evaluated for verotoxin (VT) gene screening from food materials. The pure culture and foods such as sliced beef, tandoori paste, cucumber, etc. were used for this study. The sensitivity was...

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Published inFood Hygiene and Safety Science (Shokuhin Eiseigaku Zasshi) Vol. 62; no. 4; pp. 129 - 132
Main Authors Mori, Tetsuya, Kishino, Kanae, Tahara, Maiko, Moriyama, Takatoshi, Wada, Shintaro, Ito, Takeshi
Format Journal Article
LanguageJapanese
English
Published Tokyo Japanese Society for Food Hygiene and Safety 25.08.2021
Japan Science and Technology Agency
Subjects
Assaying
Detection
Enrichment
Food
Foods
isothermal amplification assay
Pure culture
Screening
Sensitivity
Shiga toxin
Shiga toxin-producing Escherichia coli
Toxins
Verotoxin
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Abstract The sensitivity of the 3M TM Molecular Detection Assay 2-STEC Gene Screen (stx) assay (3M MDA2 STEC assay) was evaluated for verotoxin (VT) gene screening from food materials. The pure culture and foods such as sliced beef, tandoori paste, cucumber, etc. were used for this study. The sensitivity was obtained as 3 to 4 log CFU/mL in enrichment broth (BPW and mEC), which was cultured with food matrices. These results showed this detection kit was suitable the notification of standard methods from Ministry of health, which requires 4 log CFU/mL as detection limit in enrichment broth. This assay was useful as a rapid and simple screening method for VT gene from foods.
AbstractList The sensitivity of the 3M TM Molecular Detection Assay 2-STEC Gene Screen (stx) assay (3M MDA2 STEC assay) was evaluated for verotoxin (VT) gene screening from food materials. The pure culture and foods such as sliced beef, tandoori paste, cucumber, etc. were used for this study. The sensitivity was obtained as 3 to 4 log CFU/mL in enrichment broth (BPW and mEC), which was cultured with food matrices. These results showed this detection kit was suitable the notification of standard methods from Ministry of health, which requires 4 log CFU/mL as detection limit in enrichment broth. This assay was useful as a rapid and simple screening method for VT gene from foods.
Author Moriyama, Takatoshi
Kishino, Kanae
Ito, Takeshi
Mori, Tetsuya
Tahara, Maiko
Wada, Shintaro
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  organization: Incorporated Foundation Tokyo Kenbikyo-in
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Cites_doi 10.1016/j.ijfoodmicro.2016.03.031
10.5803/jsfm.34.13
10.35925/j.multi.2020.4.14
10.3389/fmicb.2014.00770
10.1080/10408398.2013.775567
10.5803/jsfm.31.194
10.5803/jsfm.32.185
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SubjectTerms Assaying
Detection
Enrichment
Food
Foods
isothermal amplification assay
Pure culture
Screening
Sensitivity
Shiga toxin
Shiga toxin-producing Escherichia coli
Toxins
Verotoxin
Title Evaluation of Simple and Rapid Shiga Toxin Gene Detection of Enterohemorrhagic Escherichia coliin Food Using 3MTM Molecular Detection Assay2-STEC Gene Screen Kit
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