Glutathione and its structural modifications recognized by Raman Optical Activity and Circularly Polarized Luminescence

• Published in: Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy Vol. 324; p. 124995
• Main Authors: Domagała, Agnieszka, Buda, Szymon, Baranska, Malgorzata, Zając, Grzegorz
• Format: Journal Article
• Language: English
• Published: Elsevier B.V 05.01.2025
• Subjects: Circularly Polarized Luminescence; Glutathione; Post-translational modifications; Raman Optical Activity; Raman Optical Activity; Post-translational modifications; Circularly Polarized Luminescence; Glutathione
• ISSN: 1386-1425
• DOI: 10.1016/j.saa.2024.124995

[Display omitted] •ROA and CPL were used in the spectral recognition of GSH peptide and its model post-translational modifications (PTMs).•The effect of pH and chirality induction of Eu(III) probes on the spectral recognition of studied peptides was discussed.•Eu(III) probes proved to be sensitive to the structural modifications of model PTMs. Raman Optical Activity combined with Circularly Polarized Luminescence (ROA-CPL) was used in the spectral recognition of glutathione peptide (GSH) and its model post-translational modifications (PTMs). We demonstrate the potential of ROA spectroscopy and CPL probes (EuCl3, Na3[Eu(DPA)3], NaEuEDTA) in the study of unmodified peptide, i.e. GSH, and its derivatives, i.e. glutathione oxidized (GSSG), S-acetylglutathione (GSAc) and S-nitrosoglutathione (GSNO). ROA spectral features of GSH, GSSG, and GSAc were determined along with thier changes upon the different pH conditions. Apart from the ROA, induced CPL signals of Eu(III) probes also proved to be sensitive to the structural modifications of GSH-based model PTMs, enabling their spectral recognition, especially by the NaEuEDTA probe.