Adenosine-dependent regulation of paracrine factors expression in human venous blood monocytes

The flow cytometry analysis of human peripheral blood monocytes separated by a two step density gradient centrifugation is reported. The expression of mRNA level of adenosine receptor (AdoR) subtypes (A1, A2A, A2B and A3) and interleukin 6 (IL-6), interleukin 8 (IL-8) and vascular endothelial growth...

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Published inBi͡u︡lletenʹ Sibirskoĭ medit͡s︡iny Vol. 10; no. 3; pp. 54 - 61
Main Authors S. V. Ryzhov, K. S. Yuryeva, K. V. Goremykin, Ye. V. Korotkaya, I. V. Saltykova, Yu. A. Yakovleva, Ye. S. Kulikov, O. S. Fyodorova, Ye. E. Kremer, N. S. Fattakhov, A. E. Sazonov
Format Journal Article
LanguageEnglish
Published Siberian State Medical University (Tomsk) 28.06.2011
Subjects
аденозин
аденозиновые рецепторы
интерлейкин-6
интерлейкин-8
моноциты
паракринные факторы
фактор роста эндотелия сосудов
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Summary:The flow cytometry analysis of human peripheral blood monocytes separated by a two step density gradient centrifugation is reported. The expression of mRNA level of adenosine receptor (AdoR) subtypes (A1, A2A, A2B and A3) and interleukin 6 (IL-6), interleukin 8 (IL-8) and vascular endothelial growth factor (VEGF) in monocytes were determined using real-time PCR. We found considerable variation across individuals in mRNA expression levels of paracrine factors after the stimulation of adenosine receptors. Our findings suggests the role of adenosinergic system accounted for interindividual differences in monocyte activation.
ISSN:1682-0363
1819-3684
DOI:10.20538/1682-0363-2011-3-54-61