A sort and sequence approach to dissect heterogeneity of response to a self-amplifying RNA vector in a novel human muscle cell line

• Published in: Molecular therapy. Nucleic acids Vol. 36; no. 1; p. 102400
• Main Authors: Barton, Rachel D., Tregoning, John S., Wang, Ziyin, Gonçalves-Carneiro, Daniel, Patel, Radhika, McKay, Paul F., Shattock, Robin J.
• Format: Journal Article
• Language: English
• Published: Elsevier Inc 11.03.2025; Elsevier
• Subjects: alphavirus; expression; innate immunity; MT: Delivery Strategies; RNA; self-amplifying; vaccine; vaccine; expression; RNA; innate immunity; alphavirus; MT: Delivery Strategies; self-amplifying

Self-amplifying RNA (saRNA) is an extremely promising platform because it can produce more protein for less RNA. We used a sort and sequence approach to identify host cell factors associated with transgene expression from saRNA; the hypothesis was that cells with different expression levels would ha...